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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 35,837 results for: CaS

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Showing: 6321 - 6340 of 35837 results

  4. pJRH029

    Plasmid
    #171060
    Purpose
    Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Insert
    Gag-spyCas9
    Use
    CRISPR and Lentiviral
    Tags
    2x SV40 NLS and 3x FLAG tag
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171060-plasmid-map-sequence-id-457342

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  13. pJRH051

    Plasmid
    #171625
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171625-plasmid-map-sequence-id-335505

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  14. pYPQ220-Ultra

    Plasmid
    #176889
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d
    Insert
    AsCas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Plant
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    176889-plasmid-map-sequence-id-350058

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  19. pXYB089

    Plasmid
    #239628
    Purpose
    Plant RNA Vision constructs with no gRNAs together with RFP input
    Depositor
    Xiaohan Yang
    Article
    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-
    Inserts
    sfGFPn_Y66-P1_loop_01-stem01
    stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    June 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239628-plasmid-map-sequence-id-477065

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  20. pXYB082

    Plasmid
    #239578
    Purpose
    Plant RNA Vision constructs with 41 bp gRNAs together with RFP input
    Depositor
    Xiaohan Yang
    Article
    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-
    Inserts
    sfGFPn_Y66-P1_loop_01-stem01-gRNA01
    gRNA02-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    June 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239578-plasmid-map-sequence-id-477039

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Showing: 6321 - 6340 of 35837 results