We narrowed to 14,359 results for: cas9

Showing: 6321 - 6340 of 14359 results

Results per page:

Sort results by:

sgRNA1_GAL4UAS-Luciferase reporter

Plasmid

#64157

Purpose

Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1 for GAL4UAS-Luciferase reporter

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pX459-sg-tdTomato90/816

Plasmid

#179918

Purpose

Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

Depositor

Indeever Madireddy, Johan Sosa

Publication

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tdTomato sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBC2101-reci

Plasmid

#117170

Purpose

U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLS

Depositor

David Savage

Publication

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_wtCas9 with SV40 NLS

Use

Lentiviral

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBC2102-reci

Plasmid

#117171

Purpose

U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLS

Depositor

David Savage

Publication

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_wtCas9 without NLS

Use

Lentiviral

Promoter

U6 Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTaU6-esgRNA

Plasmid

#115630

Purpose

expression esgRNA in wheat protoplasts

Depositor

Caixia Gao

Publication

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

TaU6p-esgRNA

Use

CRISPR

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-PpU6P-sgRNA-L1L2

Plasmid

#113735

Purpose

Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Publication

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR and Gateway Cloning

Promoter

P. patens U6 promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge
pJA50

Plasmid

#59931

Purpose

gRNA for cleavage at unc-58(e665) locus in C elegans

Depositor

Andrew Fire

Publication

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

unc-58(e665) gRNA

Use

CRISPR

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC49

Plasmid

#104823

Purpose

CRISPR/Cas9 2xplex gRNA targeting Glyma17g11235 (Dcl4a). Also expresses Cas9 from AtUBQ10 promoter.

Depositor

Robert Stupar

Insert

Glyma17g11235

Use

CRISPR

Expression

Plant

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV_Actb HR donor

Plasmid

#97317

Purpose

HR donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Publication

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HR donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pU6-Decoy

Plasmid

#80324

Purpose

Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

Depositor

Sebastian Lourido

Publication

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Decoy sgRNA

Use

CRISPR

Promoter

U6

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pAS_sfGFP150K

Plasmid

#193313

Purpose

pAS control plasmid for sfGFP expression

Depositor

Simon Elsaesser

Publication

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

super folder GFP

Expression

Mammalian

Mutation

150K in sfGFP

Promoter

EF1

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM38869

Plasmid

#153220

Purpose

Level 0 Arabidopsis U6-promoter vector

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Arabidopsis U6 snRNA promoter

Use

Synthetic Biology

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
eGFP L138 Reporter

Plasmid

#119130

Purpose

Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

Depositor

Reuben Harris

Publication

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

mCherry and eGFP

Use

Lentiviral

Mutation

eGFP L138S

Promoter

CMV

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF221

Plasmid

#121669

Purpose

U6-sgRNA EF1a-mCherry

Depositor

David Savage

Publication

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

mCherry

Use

Lentiviral

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
sg2.0 (PP7) Locus#2

Plasmid

#101155

Purpose

gRNA with two PCP binding sites

Depositor

Mazhar Adli

Publication

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

Locus #2(tandem repeat)

Use

CRISPR and Lentiviral

Promoter

hU6

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ133D

Plasmid

#69289

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA4_ASCL1

Plasmid

#64132

Purpose

Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_ASCL4 (ASCL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-puro U6 sgRNA SOX17 -177

Plasmid

#50925

Purpose

U6 driven sgRNA targeting Sox17 -177 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Publication

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Sox17 -177 sgRNA (SOX17 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBHM2406

Plasmid

#173442

Purpose

pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

Depositor

Hiten Madhani

Publication

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

mNeonGreen-CBP-2XFLAG-HYGB

Tags

CBP-2xFLAG

Expression

Bacterial and Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pORANGE GFP-Grin1 KI #3

Plasmid

#139659

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge