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pBOB-N-Flag-GK (281-473)

Plasmid

#134273

Purpose

Lentivector encoding Flag-tagged GK truncation (amino acids 281-473)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk amino acids 281-473

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-GK (12-272)

Plasmid

#134272

Purpose

Lentivector encoding Flag-tagged GK truncation (amino acids 12-272)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk amino acids 12-272

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-GK_v1

Plasmid

#134270

Purpose

Lentivector encoding Flag-tagged GK (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk isoform 1; 524 amino acids

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D443N)

Plasmid

#134293

Purpose

Expresses GK5 (isoform 2)-D443N mutant in bacteria

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acid 443 to Asparagine

Promoter

T7

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D280N)

Plasmid

#134290

Purpose

Expresses GK5 (isoform 2)-D280N mutant in bacteria

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acid 280 to Asparagine

Promoter

T7

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D30N)

Plasmid

#134289

Purpose

Expresses GK5 (isoform 2)-D30N mutant in bacteria

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acid 30 to Asparagine

Promoter

T7

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-C-Flag-GK5_v1

Plasmid

#134281

Purpose

Expresses Flag-tagged GK5 (isoform 1) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-GK5_v2

Plasmid

#134280

Purpose

Expresses GK5 (isoform 2) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-GK5_v1

Plasmid

#134279

Purpose

Expresses GK5 (isoform 1) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-GK_v2

Plasmid

#134271

Purpose

Lentivector encoding Flag-tagged GK (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk isoform 2; 553 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-3HA-GK5_v2

Plasmid

#134269

Purpose

Lentivector encoding 3XHA-tagged GK5 (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-3HA-GK5_v1

Plasmid

#134268

Purpose

Lentivector encoding 3XHA-tagged GK5 (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5_v2

Plasmid

#134260

Purpose

Lentivector encoding Flag-tagged GK5 (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5_v1

Plasmid

#134257

Purpose

Lentivector encoding Flag-tagged GK5 (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-eGFP-FRT-T0-RNF168 delta MIU1/MIU2

Plasmid

#133980

Purpose

inducible mammalian expression vector of eGFP tagged RNF168 where both motifs interacting with ubiquitin have been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Tags

eGFP

Expression

Mammalian

Mutation

deletion of MIU1 and MIU2

Promoter

CMV

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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mature ISG15 C-term. Domain (79-165, W123A)

Plasmid

#110765

Purpose

Bacterial expression of mutant proISG15 C-term. domain

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

proISG15 C-term. domain (79-165, W123A) (ISG15 Human)

Tags

N-His6-3C

Expression

Bacterial

Mutation

W123A

Promoter

T7

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015

Plasmid

#107578

Purpose

B. megaterium DSM319 fbp promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

Depositor

Paul Freemont

Article

Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

Insert

GFP+

Use

Synthetic Biology

Tags

B. megaterium DSM319 xylA leader sequence (MTSSKI…

Expression

Bacterial

Mutation

F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

Promoter

fbp promoter Bacillus megaterium DSM319

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-α-cateninWT

Plasmid

#229709

Purpose

Transient expression of GFP-alpha-cateninWT in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-EF1A>Tet3G:IRES:Neo

Plasmid

#198756

Purpose

Vector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Tet3G, IRES, Neo

Use

Lentiviral

Promoter

EF1A

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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hKCNQ1 pCDNA3.1(-)

Plasmid

#219953

Purpose

Expresses human KCNQ1 in mammalian cells

Depositor

Niels Decher

Article

Kiper et al Proc Natl Acad Sci U S A. 2024 Jun 18;12

Insert

KCNQ1 (KCNQ1 Human)

Expression

Mammalian

Mutation

contains silent sequence variations

Promoter

CMV

Available Since

Aug. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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