We narrowed to 15,674 results for: NOL

1. YPRCΔ15α1

   Plasmid

   #101712

   Purpose

   GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Type

   Empty backbone

   Use

   Yeast expression

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G03 IL2

   Plasmid

   #67053

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   IL2 (IL2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pOCC114

   Plasmid

   #118904

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with TEV protease, two glycines

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. CP1300-ABEmax

   Plasmid

   #119810

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)
   

   Expression

   Mammalian

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX601‐mhCMV‐miniABEmax‐N2‐ E53ogRNA

   Plasmid

   #187065

   Purpose

   Gp41-1 Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

   Depositor

   Renzhi Han

   Article

   Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

   Insert

   miniABEmax-Gp41-1
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   mhCMV

   Available Since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX601‐mhCMV‐ABEmaxNGA‐C2‐ E53ogRNA

   Plasmid

   #187066

   Purpose

   Gp41-1 Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

   Depositor

   Renzhi Han

   Article

   Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

   Insert

   Gp41-1-ABEmaxNGA
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   mhCMV

   Available Since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. TAL2385

   Plasmid

   #36794

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   BRCA1-TALEN_Right (Brca1 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pML3009

   Plasmid

   #193256

   Purpose

   Gamma-aminobutyric acid sensor expressing eYFP reporter

   Depositor

   Lauren Andrews

   Article

   Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

   Inserts

   gabR
   eYFP
   

   Use

   Synthetic Biology

   Promoter

   synthetic promoter P2 and synthetic promoter PGab…

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-hPDIb′a′

   Plasmid

   #60556

   Purpose

   enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

   Depositor

   Han Choe

   Article

   Song et al Appl Biochem Biotechnol. 2013 May;170(1)

   Type

   Empty backbone

   Tags

   His6-hPDIb'a' tag

   Expression

   Bacterial

   Available Since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pASKt15C+SrtA5

    Plasmid

    #64985

    Purpose

    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET16b-Trx C35S

    Plasmid

    #36947

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin-1 C35S mutation (TXN Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    cys 35 to serine

    Promoter

    T7

    Available Since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pconst #4-RFP

    Plasmid

    #81078

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Promoter

    Constitutive Promoter - BBa_J23107

    Available Since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. YPRCΔ15Ω1

    Plasmid

    #101710

    Purpose

    GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Type

    Empty backbone

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCLR2070

    Plasmid

    #28063

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains N496D point mutation in FokI domain, as …

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCLR2068

    Plasmid

    #28062

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains H537R point mutation in FokI domain, as …

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEU3-NII-FLAGLICNot

    Plasmid

    #140184

    Purpose

    Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, FLAG-tag and NotI restriction site

    Depositor

    Tamás Mészáros

    Article

    Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

    Type

    Empty backbone

    Use

    Expression vector for a cell-free system

    Tags

    FLAG

    Promoter

    T7

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQnl_Pcphy23

    Plasmid

    #191354

    Purpose

    Clostridium plasmid expressing NanoLuc reporter from moderate, constitutive promoter

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Pcphy23-NanoLuc
    

    Expression

    Bacterial

    Promoter

    Pcphy23

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pconst #1-RFP

    Plasmid

    #81075

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Promoter

    Constitutive Promoter - BBa_J23119

    Available Since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSV(X) C/EBPbeta

    Plasmid

    #13393

    Depositor

    Richard Schwartz

    Article

    Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

    Insert

    C/EBPbeta (Cebpb Rat)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pCfB6677

    Plasmid

    #106155

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6632, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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