We narrowed to 9,499 results for: pho;

1. pET24a_UDP_Xase_E162

   Plasmid

   #73160

   Purpose

   Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E162A

   Depositor

   Sandra Gabelli

   Article

   Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

   Insert

   pET24a
   

   Expression

   Bacterial

   Mutation

   change glutamate 162 to alanine

   Available Since

   March 22, 2016

   Availability

   Academic Institutions and Nonprofits only

2. miR-451 Trap

   Plasmid

   #61487

   Purpose

   to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoter

   Depositor

   Curt Civin

   Article

   Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

   Insert

   miR-451 Trap
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   bidirectional phosphoglycerate kinase promoter (b…

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pmCherry_C1 I3

   Plasmid

   #61398

   Purpose

   Expresses mCherry-tagged Inhibitor-3 in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

   Insert

   Inhibitor-3 (PPP1R11 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA5FRT/TO-GFP-I3

   Plasmid

   #61401

   Purpose

   Expresses GFP-tagged Inhibitor-3 in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

   Insert

   Inhibitor-3 (PPP1R11 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pNB786

   Plasmid

   #60151

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus
   

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   LEU1pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Danio rerio cryaa V62T

   Plasmid

   #47630

   Purpose

   Recombinant protein production

   Depositor

   Mason Posner

   Article

   Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

   Insert

   Alpha A-crystallin (cryaa Zebrafish)
   

   Expression

   Bacterial

   Mutation

   Changed Valine 62 to Threonine

   Promoter

   T7

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Danio rerio cryaa T147V

   Plasmid

   #47628

   Purpose

   Recombinant protein production

   Depositor

   Mason Posner

   Article

   Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

   Insert

   Alpha A-crystallin (cryaa Zebrafish)
   

   Expression

   Bacterial

   Mutation

   Changed Theronine 147 to Valine

   Promoter

   T7

   Available Since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. Danio rerio cryaa C143S

   Plasmid

   #47619

   Purpose

   Recombinant protein production

   Depositor

   Mason Posner

   Article

   Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

   Insert

   Alpha A-crystallin (cryaa Zebrafish)
   

   Expression

   Bacterial

   Mutation

   Cysteine 143 to Serine

   Promoter

   T7

   Available Since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEG593

   Plasmid

   #40119

   Depositor

   Geraldine Seydoux

   Article

   Griffin et al Cell. 2011 Sep 16;146(6):955-68.

   Insert

   Mex-5 (ATG through 648 bp of 3'UTR) R274E K318E (RNAi res) (mex-5 Nematode)
   

   Use

   Gateway destination vector

   Tags

   Dendra2 PAFP (sequence optimized for worm express…

   Expression

   Worm

   Mutation

   R274E, K318E, Exon 2 recoded to be RNAi-resistant

   Promoter

   4.4 kb mex-5 promoter fragment

   Available Since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pEG628

    Plasmid

    #40120

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (aa1-244) (RNAi resistant) (mex-5 Nematode)
    

    Use

    Gateway destination vector

    Tags

    Dendra2 PAFP (sequence optimized for worm express…

    Expression

    Worm

    Mutation

    contains aa1-244, Exon 2 recoded to be RNAi-resis…

    Promoter

    4.4 kb mex-5 promoter fragment

    Available Since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pET-17b_K490clm_E215C

    Plasmid

    #169995

    Purpose

    Kinesin KIF5B K490 cysteine light mutant with E215C mutation for single cysteine labeling

    Depositor

    Ron Vale

    Article

    Niekamp et al Proc Natl Acad Sci U S A. 2019 Mar 5;116

    Insert

    kinesin family member 5B, isoform CRA_a
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C7S, C65A, C168A, C174S, E215C, C294A, C330S, C42…

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

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12. PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH

    Plasmid

    #178960

    Purpose

    Expresses saCas9 protein specifically in astrocytes

    Depositor

    Baljit Khakh

    Article

    Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

    Insert

    Staphylococcus aureus Cas9 (SaCas9)
    

    Use

    AAV

    Tags

    3x HA

    Promoter

    gfaABC1D (astrocyte specific)

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. SNAP-Sec61B

    Plasmid

    #141152

    Purpose

    Fluorescent labeling of ER membrane in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    Sec61 beta (SEC61B Human)
    

    Tags

    SNAP tag

    Expression

    Mammalian

    Mutation

    L79M on Sec61B

    Promoter

    CMV

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Voltron

    Plasmid

    #119033

    Purpose

    Express Voltron in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmPB2-VSV

    Plasmid

    #68038

    Purpose

    Expresses mouse Plexin-B2 with N-terminal VSV tag in mammalian cells

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B2 (Plxnb2 Mouse)
    

    Tags

    VSV

    Expression

    Mammalian

    Available Since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4-RI1-332-MYC-His6

    Plasmid

    #185351

    Purpose

    Mammalian expression of Ribophorin I

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    Ribophorin I (RPN1 Human)
    

    Tags

    Myc, 6xHis

    Expression

    Mammalian

    Mutation

    aa 1-332 only

    Available Since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-EGFP-Cdc42-Q61L

    Plasmid

    #12986

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 constitutively active (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. DHB-mVenus

    Plasmid

    #136461

    Purpose

    Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

    Depositor

    Tobias Meyer, Sabrina Spencer

    Article

    Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

    Insert

    DNA Helicase B (HELB Human)
    

    Use

    Lentiviral

    Tags

    mVenus

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-EGFP-Cdc42-wt

    Plasmid

    #12975

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-cofilin WT

    Plasmid

    #78295

    Purpose

    Mammalian expression of mouse cofilin fused to EGFP

    Depositor

    Michael Frotscher

    Article

    Chai et al Development. 2016 Mar 15;143(6):1029-40.

    Insert

    cofilin 1 (Cfl1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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