We narrowed to 3,209 results for: EED
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pMIR-Report-Luc-KLF4-K1S
Plasmid#62979PurposeTranslational reporter for KLF4DepositorInsertKLF4 partial sequence (KLF4 Human)
UseLuciferaseTagsfirefly luciferaseMutationFragment 1-446 of KLF4PromoterCMVAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET45b-tsr(B/X) (6X-His-Dm-Cofilin)
Plasmid#128278PurposeExpresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell cultureDepositorInserttwinstar (tsr) (tsr Fly)
Tags6X Histidine tagExpressionBacterialMutationMet1 changed to Pro1; Cys77 silent basepair mutat…PromoterT7Available SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-AURKB 3'UTR
Plasmid#29475DepositorInsertAURKB 3"UTR (AURKB Human)
ExpressionMammalianAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL3-321/+120 Ebox mut1
Plasmid#35544DepositorUseLuciferaseTagsluciferaseMutationT to G at position -96 in promoterPromoternoneAvailable SinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3-321/+120 Ebox mut2
Plasmid#35545DepositorUseLuciferaseTagsluciferaseMutationT to G at position -52 in promoterPromoternoneAvailable SinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV-FFluc-GPD1L-3'UTR Full mut
Plasmid#32489DepositorInsertGPD1L 3'UTR (Gpd1l Mouse)
TagsLuciferaseExpressionMammalianMutationMutated in the entire miR-210 binding regionPromoterCMVAvailable SinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Repo-Man-1-890
Plasmid#184272PurposeExpresses C-terminally truncated GFP-RepoManDepositorInsertcell division cycle associated 2 (CDCA2 Human)
TagsGFPExpressionMammalianMutationtruncated Repo-Man after aminoacid 893PromoterCMVAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
H2B-GFP-Aurora B KD
Plasmid#184043PurposeExpresses H2B-GFP fused to Aurora B kinase deadDepositorInsertAURKB (AURKB Human)
TagsEGFP and H2BExpressionMammalianMutationChanged Lysine 65 to ArgininePromoterCMVAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RepoMan-S893D
Plasmid#204995PurposeExpresses shRNA resistant GFP-RepoMan S893D MutantDepositorInsertcell division cycle associated 2 (CDCA2 Human)
TagsGFPExpressionMammalianMutationchanged Serine 893 to Aspartic acidPromoterCMVAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-VIM270-pCM
Plasmid#215869PurposeThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.DepositorInserttarget site which is completely complementary to passanger strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
ExpressionMammalianAvailable SinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1
Plasmid#216378PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)DepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTagsHA signal sequence-FLAGExpressionInsectPromoterPolyhedrinAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV Flag-LRRK2 K17A/K18A/R1441G
Plasmid#186013PurposeFlag-LRRK2 K17A/K18A/R1441GDepositorInsertFlag-LRRK2 K17A/K18A/R1441G (LRRK2 Human)
ExpressionMammalianMutationSee depositor's comments below.Available SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1ΔN (MT2B2)-MutTER
Plasmid#178035PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationFirst N-Terminal 27aa are deleted and Changed T(8…PromoterT7LacIAvailable SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1CAN-MutTER
Plasmid#178033PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationChanged T(108)A, E(109)A, R(110)APromoterT7LacIAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1ΔN (MT2B2)
Plasmid#178034PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationFirst N-Terminal 27aa are deletedPromoterT7LacIAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCheck Cdk2ap1ΔN (MT2B2)
Plasmid#178029Purposeexpression vector to test translation efficiency by luciferase reporter.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseLuciferase; NonviralTagsLuciferaseExpressionMammalianPromoterCloned inAvailable SinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Ala271
Plasmid#158631PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGMutationQ271A please see depositor comments belowAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Ala271 hygro
Plasmid#158637PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorAvailable SinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Gln93,Ala271 hygro
Plasmid#158638PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Gln93,Ala271
Plasmid#158632PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Ala148,Ala271
Plasmid#158633PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro
Plasmid#158639PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Ala148 hygro
Plasmid#158636PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Gln93 hygro
Plasmid#158635PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR_P4-P1R:PER36modp
Plasmid#128431PurposeGateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).DepositorInsertPEROXISADE36 (AT3G50990 Mustard Weed)
UseGateway promoter entry cloneMutationPremature stop codons introduced into the Xero1 (…PromoterPEROXISADE36Available SinceOct. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSB61 - pL0_V2 (CDS1)
Plasmid#123183PurposeGolden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005DepositorInsertTomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)
UsePart for plant expressionExpressionPlantMutationNo BpiI and BsaI sitesAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pABD823
Plasmid#67911PurposeProtein interaction with yeast two hybrid assay of CTG10 in yeast, Saccharomyces cerevisiaeDepositorInsertCold temperature germinating 10 (CTG10)
TagsGAL4 DNA binding domain (148 amino acids)ExpressionYeastPromoterpADH1 (alcohol dehydrogenase 1 promoter)Available SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pABD933
Plasmid#67917PurposeEntry clone made prior to studies of protein co-localization of CTG10 via fluorescent fusion after transient expression in plantaDepositorInsertCTG10
UseGateway cloning vectorAvailable SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAP1893-1
Plasmid#105248Purposehuman GFP11 with tagRFP 33bp downstream in Lamin A/C (recoded)DepositorInsertInsertion of GFP11 at cut tagRFP 33bp downstream (recoded *) in Lamin A/C (LMNA Human)
ExpressionBacterialAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K2S
Plasmid#62980PurposeTranslational reporter for KLF4DepositorInsertKLF4 partial sequence (KLF4 Human)
UseLuciferaseTagsfirefly luciferaseMutationFragment 446-911 of KLF4PromoterCMVAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K3S
Plasmid#62981PurposeTranslational reporter for KLF4DepositorInsertKLF4 partial sequence (KLF4 Human)
UseLuciferaseTagsfirefly luciferaseMutationFragment 911-1406 of KLF4PromoterCMVAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K4S
Plasmid#62982PurposeTranslational reporter for KLF4DepositorInsertKLF4 partial sequence (KLF4 Human)
UseLuciferaseTagsfirefly luciferaseMutationFragment 1406-1781 of KLF4PromoterCMVAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K6S
Plasmid#62983PurposeTranslational reporter for KLF4DepositorInsertKLF4 partial sequence (KLF4 Human)
UseLuciferaseTagsfirefly luciferaseMutationFragment 2086-2639 of KLF4PromoterCMVAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-EGFP-Tau
Plasmid#46904Purposeexpresses EGFP tagged Tau in mammalian cellsDepositorInsertMAPT (MAPT Human)
TagsEGFPExpressionMammalianMutationThis WT htau construct contains human four-repeat…PromoterCMVAvailable SinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast ACSL4
Plasmid#173164PurposeRetroviral vector to express sgRNA resistant human ACSL4DepositorInsertAcyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
UseRetroviralMutationSynonymous mutations at sgRNA sitesPromoterMMLVAvailable SinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV-AR-C563A-C596A
Plasmid#171220PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: mutations of one essential cysteine on each of the two zinc fingers.DepositorInsertAR-C563A-C596A (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-C563A-C596APromoterEF-1aAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV-AR-R599A-N600A
Plasmid#171221PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: R599A-N600A, mutations in the D-box of the Zinc finger 2 that mediates AR dimerization.DepositorInsertAR-R599A-N600A (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-R599A-N600APromoterEF-1aAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
M23: pHR-SFFVp-FUSN::mCherry::SspB
Plasmid#122148PurposeN terminal domain of FUS (1-214) tagged with SspB and mCherry.DepositorInsertFUS (FUS Human)
UseLentiviralTagsmCherry-sspBExpressionMammalianMutationContains only amino acids 1-214PromoterSFFVAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only
