We narrowed to 31,999 results for: Eng

1. pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

   Plasmid

   #83947

   Purpose

   gRNA cassette-carrying vector with natMX marker

   Depositor

   Irina Borodina

   Article

   Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

   Insert

   gRNA targeting ADE2 gene
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHB-2

   Plasmid

   #140955

   Purpose

   For PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.

   Depositor

   Kang Zhou

   Article

   Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

   Insert

   phaCAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R238S mutation in PhaC

   Promoter

   Available since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCOLA-Spec-yibDp-Erv1p

   Plasmid

   #212094

   Purpose

   Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   Sulfydryl oxidase (ERV1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas9-beta

   Plasmid

   #169240

   Purpose

   Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Inserts

   cas9
   beta
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS2386-Tier1-PhCMV-GCaMP6s

   Plasmid

   #169519

   Purpose

   Tier-1 vector encoding PhCMV-driven calcium sensor GCaMP6s (PhCMV-GCaMP6s-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven green fluorescent genetically encoded calcium indicator GCaMP6s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCOLA-Gent-EM7-Erv1p

   Plasmid

   #202484

   Purpose

   Expresses sulfhydryl oxidase (Erv1p) constitutively

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   EM7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCK255

   Plasmid

   #171155

   Purpose

   Expression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   I-SceI_sacB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPMVAu5 (R)

   Plasmid

   #98296

   Purpose

   Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

   Insert

   PSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM6-Amp-T7-roGFP

   Plasmid

   #202464

   Purpose

   Expresses redox sensitive GFP (roGFP) with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   reduction-oxidation sensitive green fluorescent protein
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS2365-Tier1-PhCMV-TetR

    Plasmid

    #169566

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven tetracycline repressor protein class B from transposon Tn10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCOLA-Gent-yibDp-Erv1p

    Plasmid

    #202483

    Purpose

    Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS2396-Tier1-PhCMV-4xCOX8_Ypet

    Plasmid

    #169534

    Purpose

    Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven miochondria-localized yellow fluorescent protein Ypet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pgRNAtet-phaZ

    Plasmid

    #169874

    Purpose

    Single guide RNA plasmid for Cas9 targeting phaZ in P. putida

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    sgRNA towards phaZ in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMM585-Tier1-PhCMV-Igk

    Plasmid

    #169486

    Purpose

    Tier-1 vector encoding PhCMV-driven secretion signal Igk (PhCMV-Igk-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Igk secretion signal (IGK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJT11

    Plasmid

    #98308

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

    Insert

    TRPL3ClLIS1>TRPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pgRNAkan-glpR

    Plasmid

    #169873

    Purpose

    Single guide RNA plasmid for Cas9 targeting glpR in P. putida

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    sgRNA towards glpR in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS416-Aga1p-FAPB2.3.6L1TAG

    Plasmid

    #158148

    Purpose

    Yeast display reporter for non-display yeast strains (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p-FAPB2.3.6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first position of th…

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTS2410-Tier1-PhCMV-Myr_mTagBFP2

    Plasmid

    #169535

    Purpose

    Tier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pILGFPU0 (R)

    Plasmid

    #83562

    Purpose

    Express yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    TEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVH72-Tier1-PhCMV-SEAP

    Plasmid

    #169560

    Purpose

    Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven human secreted placental alkaline phosphatase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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