We narrowed to 19,527 results for: iNOS

1. pJK048

   Plasmid

   #75230

   Purpose

   Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Article

   Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

   Promoter

   T7 and n/a

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG/ATP8B1-HA

   Plasmid

   #209209

   Purpose

   Mammalian expression of ATP8B1

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

   Insert

   ATP8B1 (ATP8B1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-hRXRα

   Plasmid

   #135910

   Purpose

   Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 Promoter

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Insert

   Human retinoid X receptor- alpha (RXRA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG/ATP8A1-HA

   Plasmid

   #209208

   Purpose

   Mammalian expression of ATP8A1

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

   Insert

   ATP8A1 (ATP8A1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBabe puro HA PIK3CA H1047R

   Plasmid

   #12524

   Purpose

   Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

   Depositor

   Jean Zhao

   Article

   Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

   Insert

   PIK3CA H1047R (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H1047R. Tumor-derived allele.

   Promoter

   Available Since

   Aug. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NLS-Laconic/pCMV-myc-nuc

   Plasmid

   #46307

   Purpose

   a nuclear-targeted, genetically-encoded sensor for lactate

   Depositor

   Luis Felipe Barros

   Article

   Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

   Insert

   NLS-Laconic
   

   Use

   Lactate biosensor

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG/ATP11C-HA

   Plasmid

   #209220

   Purpose

   Mammalian expression of ATP11C

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

   Insert

   ATP11C (ATP11C Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-NUP188

   Plasmid

   #159893

   Purpose

   Expresses NUP188 in mammalian cells

   Depositor

   Kozo Tanaka

   Article

   Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:

   Insert

   NUP188 (NUP188 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   To avoid NotI site, first three amino acids are d…

   Promoter

   CMV

   Available Since

   Oct. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBpaRS3tRNA

   Plasmid

   #197102

   Purpose

   To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

   Depositor

   Kensaku Sakamoto

   Article

   Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

   Insert

   TyrRS variant, amber suppressor tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

   Promoter

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 6His-MBP-TEV-huAsCpf1

    Plasmid

    #90095

    Purpose

    Bacterial expression plasmid for protein purification

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huAsCpf1
    

    Use

    Tags

    3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMRXIP-GFP-Spo20(PABD)

    Plasmid

    #221758

    Purpose

    Stable expression of GFP-Spo20(PABD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    Spo20 (SOS1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    tandem Spo20(PABD) (amino acids 422-551)

    Promoter

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GLuc-FLAG-CB1

    Plasmid

    #158066

    Purpose

    Membrane expression detection of Cannabinoid receptor 1

    Depositor

    Adolfo Rivero-Muller

    Article

    Rodriguez-Rodriguez et al Sci Rep. 2020 Oct 23;10(1):18191. doi: 1

    Insert

    CB1 (CNR1 Human)
    

    Use

    AAV

    Tags

    Gaussia Luciferase

    Expression

    Mammalian

    Mutation

    contains 4Kb of 3' UTR

    Promoter

    pCAG

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG/ATP10A-HA

    Plasmid

    #209215

    Purpose

    Mammalian expression of ATP10A

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP10A (ATP10A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-hCB2R

    Plasmid

    #179322

    Purpose

    Express human cannabinoid 2 receptor (GFP tagged) in mammalian cells

    Depositor

    Christian Gruber

    Article

    Tomašević et al J Biol Chem. 2024 Jun;300(6):107330. doi

    Insert

    human cannabinoid 2 receptor (CNR2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDule2-Tet2.0

    Plasmid

    #85497

    Purpose

    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044

    Insert

    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32G L65Q F108S Q109D D158S L162N

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 409 pSG5L HA RB (379-928)

    Plasmid

    #10734

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB (379-928) (RB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    amino acids 379-928

    Promoter

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pBADHISTEV_PfeE

    Plasmid

    #129120

    Purpose

    pBADHISTEV carrying pfeE for overexpression of PfeE in E. coli

    Depositor

    James Naismith

    Article

    Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

    Insert

    Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    PBAD

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIYN3

    Plasmid

    #197099

    Purpose

    To express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Biochem Biophys Res Commun. 2011 Aug 12;

    Insert

    TyrRS variant, Nap3 tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Ala70, Thr158, Ser159, Arg286 (TyrRS)

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTAMAHISTEV_PfeAQ482A

    Plasmid

    #128945

    Purpose

    expresses Q482A mutant of PfeA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

    Insert

    ferric enterobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-75), …

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG/ATP9B-HA

    Plasmid

    #209214

    Purpose

    Mammalian expression of ATP9B

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP9B (ATP9B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge