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MTOR gRNA (BRDN0001145868)

Plasmid

#76784

Purpose

3rd generation lentiviral gRNA plasmid targeting human MTOR

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MTOR (MTOR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NADK2 gRNA (BRDN0001147240)

Plasmid

#78092

Purpose

3rd generation lentiviral gRNA plasmid targeting human NADK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NADK2 (NADK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ROCK1 gRNA (BRDN0001145904)

Plasmid

#77594

Purpose

3rd generation lentiviral gRNA plasmid targeting human ROCK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ROCK1 (ROCK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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cmv hTid Short H121Q

Plasmid

#13710

Depositor

Karl Munger

Article

Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

Insert

hTid Short H121Q (DNAJA3 Human, hTid Short H121Q)

Expression

Mammalian

Mutation

H121Q

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
PRKAR1A gRNA (BRDN0001146329)

Plasmid

#77720

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR1A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR1A (PRKAR1A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pExpreS2-1-CR-PA-10H-SIII

Plasmid

#175447

Purpose

HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

Depositor

Wyatt Yue

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Tags

BiP Secretion Peptide and Thrombin-Protein A-10Hi…

Expression

Insect

Promoter

Fused Actin-HSP70

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-nsp8

Plasmid

#168957

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-nsp8 (HCoV229Egp1 )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-nsp8

Plasmid

#168897

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-nsp8 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-1-nsp8

Plasmid

#168850

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-nsp8 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_MERS-CoV-nsp8

Plasmid

#168821

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-nsp8 (orf1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-C/EBPbZIP (pGEX-bZIPa)

Plasmid

#12435

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Insert

68 a.a. fragment of C/EBP alpha (CEBPA Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
PDGFRB gRNA (BRDN0001147806)

Plasmid

#77059

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDGFRB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDGFRB (PDGFRB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PLK1 gRNA (BRDN0001144995)

Plasmid

#76459

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PLK1 (PLK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ROCK1 gRNA (BRDN0001146798)

Plasmid

#77595

Purpose

3rd generation lentiviral gRNA plasmid targeting human ROCK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ROCK1 (ROCK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-SV40P-Tet1 enhancer E

Plasmid

#63880

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 enhancer fragment E

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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PRKD2 gRNA (BRDN0001147099)

Plasmid

#77614

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKD2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKD2 (PRKD2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RPS6KA5 gRNA (BRDN0001146427)

Plasmid

#77373

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA5 (RPS6KA5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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PRKD1 gRNA (BRDN0001144834)

Plasmid

#77230

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKD1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKD1 (PRKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPPAT-11AA-2XStrep

Plasmid

#125680

Purpose

expresses human PPAT protein, with first 11 residues removed, with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

PPAT (PPAT Human)

Tags

2X Strep-Tag II

Expression

Mammalian

Mutation

the first eleven residues have been removed

Promoter

CMV

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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NUDT16

Plasmid

#155794

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

NUDT16 (NUDT16 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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