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pME-AKT-PH

Plasmid

#67674

Purpose

Gateway entry clone containing AKT-PH with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

AKT-PH

Use

Gateway multisite middle entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP

Plasmid

#103815

Purpose

Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN (CIB1 Mustard Weed)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSicoR-shS6K1/2

Plasmid

#81088

Purpose

expresses an shRNA targeting both S6K1 and S6K2 (mouse)

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

S6K1 & S6K2 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGL2 p15 4xSBR1

Plasmid

#15706

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

p15 4xSBR1 (CDKN2B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pGL2 p15 4xSBR2

Plasmid

#15709

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

p15 4xSBR2 (CDKN2B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
3XFlag DVL2 (E499-G)

Plasmid

#24803

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

DVL2 (DVL2 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

E499G

Available Since

June 22, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pIS1 IMP-1 long UTR

Plasmid

#21640

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 UTR (IGF2BP1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pCIBN-mCherry

Plasmid

#103816

Purpose

Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN (CIB1 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-A2BP1

Plasmid

#16176

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Ataxin2-binding protein 1 (RBFOX1 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pGL3E-hIL-17prom(-159)-Luc

Plasmid

#25629

Depositor

Sarah Gaffen

Article

Liu et al J Biol Chem. 2004 Dec 10. 279(50):52762-

Insert

human IL-17 promoter (IL17A Human)

Use

Luciferase

Available Since

June 25, 2010

Availability

Academic Institutions and Nonprofits only
KA1868_pGL4-UBCmin-luc2/Utf1-A'

Plasmid

#124194

Purpose

Luciferase reporter driven by the Utf1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Utf1 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-neo largeTcDNA H42Q

Plasmid

#9048

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Mutation

H42Q. Renders J domain nonfunctional.

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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aL-mEos3.2

Plasmid

#113154

Purpose

constrained LFA-1-mEos3.2 fusion

Depositor

Timothy Springer

Article

Moore et al Cell Rep. 2018 Feb 13;22(7):1903-1912. d

Insert

full-length human aL with mEOS3.2 in betapropeller (ITGAL Human)

Tags

mEOS3.2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgMPC2_7

Plasmid

#163457

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 7 targeting GPT2 (MPC2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgMPC2_9

Plasmid

#163458

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 9 targeting GPT2 (MPC2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_Notch1 del-NRR

Plasmid

#188353

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-del-NRR-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Notch1-NRRdeletion-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1V5-His Snk/Plk2 D223N

Plasmid

#22218

Depositor

Wafik El-Deiry

Article

Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

Insert

Snk/Plk2 (PLK2 Human)

Expression

Mammalian

Mutation

single amino acid substitution mutant, aspartic a…

Available Since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only
pPN217

Plasmid

#91676

Purpose

Express sgRNA targeting human TLE1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

TLE1 (TLE1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-ULK2(K39T)

Plasmid

#38206

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

Unc-51 like kinase 2 (Ulk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed Lysine 39 to Threonine

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pECFP-N2-COL1A1

Plasmid

#66603

Purpose

Expresses procollagen 1 alpha 1 as a CFP fusion

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

Insert

COL1A1 (COL1A1 Human)

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only