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pET - FnR062.Y78A

Plasmid

#84784

Purpose

bacterial expression of FnR.0.6.2-Y78A protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78A

Tags

6xHis

Expression

Bacterial

Mutation

Y78A

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78C

Plasmid

#84785

Purpose

bacterial expression of FnR.0.6.2-Y78C protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78C

Tags

6xHis

Expression

Bacterial

Mutation

Y78C

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78E

Plasmid

#84787

Purpose

bacterial expression of FnR.0.6.2-Y78E protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78E

Tags

6xHis

Expression

Bacterial

Mutation

Y78E

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78L

Plasmid

#84793

Purpose

bacterial expression of FnR.0.6.2-Y78L protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78L

Tags

6xHis

Expression

Bacterial

Mutation

Y78L

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78M

Plasmid

#84794

Purpose

bacterial expression of FnR.0.6.2-Y78M protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78M

Tags

6xHis

Expression

Bacterial

Mutation

Y78M

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78N

Plasmid

#84795

Purpose

bacterial expression of FnR.0.6.2-Y78N protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78N

Tags

6xHis

Expression

Bacterial

Mutation

Y78N

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78Q

Plasmid

#84797

Purpose

bacterial expression of FnR.0.6.2-Y78Q protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78Q

Tags

6xHis

Expression

Bacterial

Mutation

Y78Q

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78T

Plasmid

#84800

Purpose

bacterial expression of FnR.0.6.2-Y78T protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78T

Tags

6xHis

Expression

Bacterial

Mutation

Y78T

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pCuHA-SpAtg101(414)

Plasmid

#122081

Purpose

Expressing S. pombe HA-Atg101

Depositor

Daniel Klionsky

Article

Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

Insert

SpATG101

Tags

3HA

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHMGWA-Cas1

Plasmid

#122285

Purpose

Cas1 cloned in pHMGWA

Depositor

Jennifer Doudna

Article

Nunez et al Nat Struct Mol Biol. 2014 Jun;21(6):528-

Insert

Eco Cas1

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_mTFP1-mVenus

Plasmid

#118859

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

mTFP1-mVenus

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pPGK1-(AscI)-λN-T2A-SpHIS5 (pNTI347)

Plasmid

#115429

Purpose

Selection for in-frame fusion expression constructs

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Type

Empty backbone

Tags

T2A-SpHIS5 and λN

Expression

Yeast

Promoter

PGK1

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) AcAc

Plasmid

#111332

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

3' splice site AcAc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
puC19 preACT1(WTdel6)

Plasmid

#111324

Purpose

for T7 transcription of wt actin pre-mRNA (del6)

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(AcAc)

Plasmid

#111327

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

3' splice site AcAc mutation in intron

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
puC19 preACT1(BrG)

Plasmid

#111326

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

branch site A to G mutation in intron

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(BrC)

Plasmid

#111325

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

branch site A to C mutation in intron

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pS1GG

Plasmid

#108197

Purpose

S. solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

S. solfataricus PCNA1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pS3GG

Plasmid

#108199

Purpose

S. solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

S. solfataricus PCNA3

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pS2GG

Plasmid

#108198

Purpose

S. solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

S. solfataricus PCNA2

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge