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HCKan-P-CYC1

Plasmid

#65338

Purpose

construction of CYC1 promoter in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1 promoter

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223 PAK5 P50L

Plasmid

#60533

Purpose

Donor vector for human Pak5 P50L mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

P50L

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 P76T

Plasmid

#60534

Purpose

Donor vector for human Pak5 P76T mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

P76T

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 S312C

Plasmid

#60535

Purpose

Donor vector for human Pak5 S312C mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

S312C

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 V463L

Plasmid

#60536

Purpose

Donor vector for human Pak5 V463L mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

V463L

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 V593I

Plasmid

#60539

Purpose

Donor vector for human Pak5 V593I mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

V593I

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
Cs2+Etv4 S100D

Plasmid

#49094

Purpose

expression of Etv4 S100D mutant

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv4 S100D (etv4 Zebrafish)

Use

Xenopus/avian/zebrafish

Expression

Mammalian

Mutation

S100D

Promoter

CMV

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pre-associated oBC-minP-GFP-mBC backbone (p043)

Pooled Library

#194098

Purpose

pre-associated oBC-minP-GFP-mBC backbone (p043), can directly be used to deposit library of regulatory elements

Depositor

Jay Shendure

Article

Lalanne et al bioRxiv 2022.12.10.519236

Expression

Bacterial and Mammalian

Use

RNAi

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only
scQers promoter control #1 (low: minimal promoter, p027)

Pooled Library

#217420

Purpose

scQers promoter control #1 (low: minimal promoter, p027)

Depositor

Jay Shendure

Article

Lalanne et al bioRxiv 2022.12.10.519236

Expression

Bacterial and Mammalian

Use

RNAi

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only
scQers promoter control #5 (high: EEF1A1 promoter, p028)

Pooled Library

#217424

Purpose

scQers promoter control #5 (high: EEF1A1 promoter, p028)

Depositor

Jay Shendure

Article

Lalanne et al bioRxiv 2022.12.10.519236

Expression

Bacterial and Mammalian

Use

RNAi

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only
scQers promoter control #3 (intermediate: UBC promoter, p041)

Pooled Library

#217422

Purpose

scQers promoter control #3 (intermediate: UBC promoter, p041)

Depositor

Jay Shendure

Article

Lalanne et al bioRxiv 2022.12.10.519236

Expression

Bacterial and Mammalian

Use

RNAi

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only
scQers promoter control #4 (intermediate: Pgk1 promoter, p042)

Pooled Library

#217423

Purpose

scQers promoter control #4 (intermediate: Pgk1 promoter, p042)

Depositor

Jay Shendure

Article

Lalanne et al bioRxiv 2022.12.10.519236

Expression

Bacterial and Mammalian

Use

RNAi

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-HaloAKAR2.2-T2A-EGFP

Plasmid

#216490

Purpose

Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.2

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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P022-mPB-TRE-PEmax-T2A-mCherry-PGK-rttA-Puro

Plasmid

#239974

Purpose

PiggyBac plasmid for inducible PEmax expression and Puromycin selection

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20

Plasmid

#44012

Purpose

Tet-inducible lentiviral vector for ORF expression

Depositor

Stephen Elledge

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Type

Empty backbone

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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Integrin β3-GFP

Plasmid

#205091

Purpose

Express GFP-tagged human integrin β3 in mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

Integrin beta 3 (ITGB3 Human)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pINDUCER dCas9-TET1CD

Plasmid

#101921

Purpose

The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

dCas9-TET1CD

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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iMAP-61

Plasmid

#187460

Purpose

gRNA array of iMAP-61

Depositor

Tian Chi

Article

Liu et al Cell. 2022 Aug 4;185(16):3008-3024.e16.

Insert

sgRNA array

Use

Piggybac

Expression

Mammalian

Promoter

modified U6

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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hLRP1-pEZT-BM

Plasmid

#177985

Purpose

Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells

Depositor

Lora Hooper

Article

Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

Insert

LDL receptor related protein 1 (LRP1 Human)

Tags

No

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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VPS13C^mClover3

Plasmid

#118760

Purpose

VPS13C internally tagged with mClover3 at residue 1914.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13C (VPS13C Human)

Tags

mClover3 inserted at position aa 1914

Expression

Mammalian

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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