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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,392 results for: crispr grnas

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Showing: 6541 - 6560 of 8392 results

  3. eSpCas9-plus

    Plasmid
    #126767
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126767-plasmid-map-sequence-id-410890

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  4. pPbU6_hdhfr/yfcu_Cas9

    Plasmid
    #216423
    Purpose
    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.
    Depositor
    Ellen Bushell
    Article
    Jonsdottir et al bioRxiv 2024.04.20.590404
    Type
    Empty backbone
    Use
    CRISPR; Expression in plasmodium berghei
    Expression
    Bacterial
    Available Since
    April 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216423-plasmid-map-sequence-id-431289

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  5. tRNA-gRNA position [M1_n-1] (GB1210)

    Plasmid
    #75411
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [M1_n-1]
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75411-plasmid-map-sequence-id-211947

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  6. pLKO Cre v4 trcr filler

    Plasmid
    #256774
    Purpose
    lentiviral construct with Cre recombinase and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
    Depositor
    Daniel Schramek
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    256774-plasmid-map-sequence-id-513565

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  7. pLKO H2BmRFP-2A-puro Plgrkt-HA v4 trcr filler

    Plasmid
    #256787
    Purpose
    lentiviral construct with H2BmRFP-2A-puromycin, Plgrkt-HA, and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
    Depositor
    Daniel Schramek
    Insert
    Plgrkt (Plgrkt Mouse)
    Use
    Lentiviral
    Tags
    HA
    Available Since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    256787-plasmid-map-sequence-id-513566

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  8. pLKO H2BmRFP-2A-Plgrkt K147R v4 trcr filler

    Plasmid
    #256783
    Purpose
    lentiviral construct with H2BmRFP-2A-Plgrkt (mouse) K147R and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
    Depositor
    Daniel Schramek
    Insert
    Plgrkt (Plgrkt Mouse)
    Use
    Lentiviral
    Mutation
    K147R
    Available Since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    256783-plasmid-map-sequence-id-513540

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  9. PEA1

    Plasmid
    #176527
    Purpose
    Delivers all prime editing nickase components in a single plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CbH for Cas9, hU6 for gRNAs
    Available Since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176527-plasmid-map-sequence-id-350558

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  14. pOSR1.1.0-gDNA

    Plasmid
    #176381
    Purpose
    Expresses spCas9 and gRNA sequence targeting OSR1
    Depositor
    Barbara Stranger, Kevin White
    Insert
    OSR1 gRNA
    Use
    CRISPR
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176381-plasmid-map-sequence-id-347393

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  18. pZIC3.1.0-gDNA

    Plasmid
    #176379
    Purpose
    Expresses spCas9 and gRNA sequence targeting ZIC3
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZIC3 gRNA
    Use
    CRISPR
    Available Since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    176379-plasmid-map-sequence-id-347383

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  19. pOTX2.1.0-gDNA

    Plasmid
    #176376
    Purpose
    Expresses spCas9 and gRNA sequence targeting OTX2
    Depositor
    Barbara Stranger, Kevin White
    Insert
    OTX2 gRNA
    Use
    CRISPR
    Available Since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    176376-plasmid-map-sequence-id-347362

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Showing: 6541 - 6560 of 8392 results