We narrowed to 19,133 results for: grna

1. pX459-HypaCas9-mR2-TUBA1B_sgRNA

   Plasmid

   #183888

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   TUBA1B sgRNA spacer (TUBA1B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-HypaCas9-mR2-RHOA_sgRNA

   Plasmid

   #183877

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   RHOA sgRNA spacer (RHOA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX458_miR-290-295KO-gRNA1

   Plasmid

   #172709

   Purpose

   Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster

   Depositor

   Constance Ciaudo

   Article

   Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

   Insert

   miR-290-295
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458_miR-290-295KO-gRNA2

   Plasmid

   #172710

   Purpose

   Cas9 with 3' gRNA for paired CRISPR KO of miR-290-295 cluster

   Depositor

   Constance Ciaudo

   Article

   Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

   Insert

   miR-290-295
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(BCL11B-E1(14))-PGKpuro2ABFP-W

   Plasmid

   #208554

   Purpose

   Lentiviral vector expressing gRNA targeting human BCL11B-E1

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   BCL11B-E1(14) (BCL11B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc-sgRNA-Cas9.scr_2

   Plasmid

   #190704

   Purpose

   Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Scrambled sgRNA 2
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila U6

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lentiviral sgRNA human CD19

   Plasmid

   #155289

   Purpose

   Lentiviral expression of sgRNA against human CD19

   Depositor

   Martin Carroll

   Insert

   CD19 (CD19 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAs-Cpf1-pGL3-U6-sgRNA

   Plasmid

   #107683

   Purpose

   Expresses AsCpf1 crRNA in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV Gsk3 sgRNA/GFP

   Plasmid

   #112733

   Purpose

   Gsk3b targeting gRNA cloned into px552 (SpGuide) plasmid.

   Depositor

   Martin Beaulieu

   Article

   Khlghatyan et al Front Mol Neurosci. 2018 Apr 12;11:119.

   Insert

   GFP
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(Nprl2-g1)-PGKpuroBFP-W

    Plasmid

    #105037

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nprl2 (Nprl2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6gRNA5(Nprl2-g2)-PGKpuroBFP-W

    Plasmid

    #105038

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nprl2 (Nprl2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO1-puro-U6-sgRNA-TGM2

    Plasmid

    #69239

    Purpose

    U6 driven SpCas9 sgRNA expression for TGM2 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TGM2 sgRNA (TGM2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pS23.U6<ActB.mus_C-term-KI_gRNA

    Plasmid

    #207408

    Purpose

    gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

    Depositor

    Alexandros Poulopoulos

    Article

    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

    Insert

    ActB
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC-U63-tgRNA-nlsBFP

    Plasmid

    #169029

    Purpose

    gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

    Depositor

    Chun Han

    Article

    Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBbdCas9S(-10AC12)_Psyn-sgRNArodA

    Plasmid

    #149661

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10AC12), Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX601‐mhCMV‐ABEmaxNGA‐C3‐ E53ogRNA

    Plasmid

    #187063

    Purpose

    Npu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    Npu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mhCMV

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSico_U6-Pan-PGC1a sgRNA

    Plasmid

    #165426

    Purpose

    Expression of gRNA against human Total PGC-1a variants

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    gRNA against human Total PGC-1a variants (PPARGC1A Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-U6-CB2gRNA-CBh-mCherry

    Plasmid

    #91948

    Purpose

    Expression of gRNA for mouse CB2 cannabinoid receptor and mCherry

    Depositor

    Jimok Kim

    Article

    Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. AeU6 3p3 GFP LgRNA

    Plasmid

    #182208

    Purpose

    For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegypti

    Depositor

    Craig Montell

    Article

    Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

    Insert

    3xP3-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

    Plasmid

    #149588

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi flaB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAflaB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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