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AP2u2-CMV-S_protein

Plasmid

#226456

Purpose

Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification

Depositor

Urartu Seker

Article

Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(

Insert

Spike Protein

Tags

6xHis Tag, Foldon Domain, and Thrombin Cleavage S…

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-E690Kmut_ABCA3-GFP

Plasmid

#188549

Purpose

Lentiviral vector allowing for EF1aL-driven constitutive expression of E690K mutant human ABCA3:GFP fusion protein

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

ABCA3:GFP (ABCA3 Human)

Use

Lentiviral

Tags

GFP

Mutation

amino acid E690->K690 (ABCA3 gene)

Promoter

EF1aL

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-W308Rmut_ABCA3-GFP

Plasmid

#188550

Purpose

Lentiviral vector allowing for EF1aL-driven constitutive expression of W308R mutant human ABCA3:GFP fusion protein

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

ABCA3 (ABCA3 Human)

Use

Lentiviral

Tags

GFP

Mutation

amino acid W308->R308 (ABCA3 gene)

Promoter

EF1aL

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_FKBP-V5-PRRX1

Plasmid

#215024

Purpose

AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human PRRX1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

paired related homeobox 1 (PRRX1 Human)

Use

AAV

Tags

FKBP-V5

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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GRPR-DuET

Plasmid

#213306

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GRPR (GRPR Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pT22UbGfpnE4PCh(#301)

Plasmid

#184066

Purpose

cyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Zhang et al Chembiochem. 2018 Jun 18;19(12):1232-123

Insert

eGFP-nls-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

Plasmid

#62580

Purpose

Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Mutation

miR-21 binding site 1 mutant (TAAGCTA --> TAGA…

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

Plasmid

#62581

Purpose

Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Mutation

miR-21 binding site 2 mutant (TAAGCTA --> TAGA…

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pEMS1710

Plasmid

#29296

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple238 (TRH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-N-HA-NEK7 K64M

Plasmid

#75143

Purpose

Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lys 64 mutated to Met; kinase inactive and F236L …

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-hATG7CS

Plasmid

#87868

Purpose

Express a active-site mutant human Atg7/Apg7 C572S in mammalian cells

Depositor

Eiki Kominami, Isei Tanida

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

ATG7 C572S mutant (ATG7 Human)

Expression

Mammalian

Mutation

C572S mutant

Promoter

CMV

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Lrp6

Plasmid

#123595

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

low density lipoprotein receptor-related protein 6 (Lrp6 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET32bplus_trxAChEwt

Plasmid

#83916

Purpose

Encodes an E. coli codon optimized version of wild type human acetylcholinesterase

Depositor

Sarel Fleishman

Article

Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:

Insert

Short form of hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)

Tags

His, S-tag, and TRX

Expression

Bacterial

Mutation

codon optimised for E.coli expression

Promoter

T7 promoter

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast mAtg7

Plasmid

#173170

Purpose

Retroviral vector to express sgRNA resistant mouse Atg7

Depositor

Kivanc Birsoy

Article

Zhu et al Cell Metab. 2020 Oct 28. pii: S1550-4131

Insert

Autophagy Related 7 (Atg7 Mouse)

Use

Retroviral

Mutation

Synonymous mutations at sgRNA sites

Promoter

MMLV

Available Since

Feb. 2, 2022

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV3D

Plasmid

#224445

Purpose

Rep/Cap plasmid for the production of MyoAAV 3D, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYREL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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CRHR1-DuET

Plasmid

#213215

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CRHR1 (CRHR1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

Plasmid

#140198

Purpose

Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

dLbCas12a(D156R)-VPR

Use

CRISPR and Synthetic Biology; Expression in asper…

Tags

VPR (VP64-p65-Rta), NLS

Mutation

D832A DNase deactivated, D156R for improved activ…

Promoter

PgpdA

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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AAV8-hSyn-flex-miR30-eGFP-shCrh

Plasmid

#132715

Purpose

Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Crh gene which encodes corticotropin releasing factor

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

GFP-shCrh (Crh Rat)

Use

AAV

Promoter

Synapsin 1

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-AP1S3

Plasmid

#58292

Purpose

mammalian expression of AP1S3

Depositor

Francesca Capon

Article

Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

Insert

AP1S3 (AP1S3 Human)

Tags

6xHis and myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pBS L30 Flag-Apex-Rab7A

Plasmid

#135652

Purpose

Rab7A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Article

Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

Insert

Rab7A (RAB7A Human)

Tags

Apex2 and FLag

Expression

Mammalian

Promoter

L30

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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