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pXR002: EF1a-dCasRx-2A-EGFP

Plasmid

#109050

Purpose

Catalytically inactive dCasRx with 2A-EGFP

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Insert

dCasRx

Use

CRISPR and Lentiviral

Tags

2A-eGFP, HA, and NLS

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-n1-APP

Plasmid

#69924

Purpose

mammalian expression of human APP fused to EGFP

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Insert

APP (APP Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDL278_P23-DsRed-Express2

Plasmid

#121505

Purpose

E. coli-streptococcal shuttle vector that expresses rfp

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

DsRed-Express2

Use

E. coli streptococcal shuttle vector

Promoter

P23

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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secreted BirA-Flag

Plasmid

#64395

Purpose

Flag-tagged biotinylation enzyme for co-expression with bio-proteins

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

BirA (birA E. coli)

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-Flag-ER-Hoxb8-IRES-GFP

Plasmid

#222292

Purpose

Less commonly used plasmid for generating ER-Hoxb8 conditionally immortalized myeloid cell lines.

Depositor

Mark Kamps, David Sykes

Article

Wang et al Nat Methods. 2006 Apr;3(4):287-93.

Insert

ER-Hoxb8 (ESR1, Hoxb8 Mouse, Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

The estrogen receptor hormone binding domain cont…

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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PC1575

Plasmid

#232088

Purpose

Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.

Depositor

Pranam Chatterjee

Article

Bhat et al Sci Adv. 2025 Jan 24;11(4):eadr8638. doi

Insert

uAb with cloning Esp3I restriction site

Tags

IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pNF02-mLychee

Plasmid

#219400

Purpose

Constitutive expression of mLychee in E. coli

Depositor

Christian Lesterlin

Article

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mLychee

Expression

Bacterial

Promoter

proDp

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHL-FcHis

Plasmid

#99846

Purpose

mammalian expression with secretive signal and FC tag

Depositor

Edith Yvonne Jones

Article

Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

Insert

ephrin b2 (EPHB2 Human)

Tags

Fc_His

Expression

Mammalian

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pJL1-mTagBFP2

Plasmid

#102638

Purpose

In vitro expression of mTagBFP2 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mTagBFP2

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3 Gag-imCherry

Plasmid

#208882

Purpose

codes for Gag from HIV-1 with an internal mCherry fluorescent protein

Depositor

Raphael Gaudin

Article

Diana Brychka et al bioRxiv

Insert

HIV-1 Gag

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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