167,318 results

1. pcDNA3 HA E2F3A

   Plasmid

   #236437

   Purpose

   transient overexpression of E2F3A in mammalian cells

   Depositor

   Michele Pagano

   Article

   Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

   Insert

   E2F3A (E2F3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. FHRE-Luc

   Plasmid

   #1789

   Purpose

   Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.

   Depositor

   Michael Greenberg

   Article

   Brunet et al Cell 1999 Mar 19;96(6):857-68.

   Insert

   FHRE (FOXO3 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   3 copies of FRE

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHLUM

   Plasmid

   #40276

   Depositor

   Markus Ralser

   Article

   Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

   Inserts

   LEU2 (LEU2 Budding Yeast)
   URA3 (URA3 Budding Yeast)
   MET15/17 (MET17 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   endogenous LEU2, endogenous MET15/17, and endogen…

   Available Since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-Etv6

   Plasmid

   #131599

   Purpose

   expression of mouse Etv6 in mammalian cells

   Depositor

   Kateri Moore, Filipe Pereira

   Article

   Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

   Insert

   Etv6 (Etv6 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPR-V2_hMSH2

   Plasmid

   #186155

   Purpose

   sgRNA targeting human MSH2 gene

   Depositor

   David McFadden

   Article

   Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

   Insert

   MSH2 (MSH2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-coilin

   Plasmid

   #36906

   Depositor

   Greg Matera

   Article

   Shpargel et al J Cell Sci. 2003 Jan 15;116(Pt 2):303-12

   Insert

   coilin (COIL Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. GPR158-Tango

   Plasmid

   #66332

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR158 (GPR158 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pLX208 CMV sTurboID (C)-HA-FRB-ERM

   Plasmid

   #153007

   Purpose

   Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID (C) (G74)-ERM (Cb5)
   

   Use

   Lentiviral

   Tags

   FRB and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDB_053

   Plasmid

   #216083

   Purpose

   for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
   

   Use

   Destination

   Expression

   Insect

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-6xGLY-3xFLAG-kanMX6

    Plasmid

    #20754

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Expression

    Yeast

    Available Since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAMD1-2

    Plasmid

    #61564

    Purpose

    Standardized mini-Tn5 delivery plasmid, kanamycin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Available Since

    Jan. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPtGE34

    Plasmid

    #107932

    Purpose

    Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

    Depositor

    David Edgell

    Article

    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

    Inserts

    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    

    Use

    CRISPR and Synthetic Biology; Episomal vector for…

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. UbcH5c WT pET28a

    Plasmid

    #12643

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
    

    Expression

    Bacterial

    Available Since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMIIS793

    Plasmid

    #238206

    Purpose

    Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    TaTasR
    

    Tags

    TwinStrepII-bdSUMO

    Expression

    Bacterial

    Available Since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. psgRNA_EcoWG1

    Pooled Library

    #131625

    Purpose

    Improved genome-wide knockdown of E. coli genes

    Depositor

    David Bikard

    Article

    Calvo-Villamanan et al Nucleic Acids Res. 2020 Apr 30. pii: 582

    Expression

    Bacterial

    Species

    Escherichia coli

    Use

    CRISPR

    Available Since

    May 12, 2020

    Availability

    Academic Institutions and Nonprofits only

16. pD649-HAsp-KIR2DL4-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156662

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIR2DL4 (KIR2DL4 Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPR-PsppA-mCherry

    Plasmid

    #225428

    Purpose

    Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

    Depositor

    Geir Mathiesen

    Article

    Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

    Insert

    mCherry, Cas9, SgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PsppA upstream of mCherry and Cas9, P3 upstream o…

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-KASH2

    Plasmid

    #187017

    Purpose

    Inducible disruption of LINC Complex

    Depositor

    Jan Lammerding

    Article

    Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

    Insert

    KASH2
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET11b-MIF

    Plasmid

    #149446

    Purpose

    Expression of WT MIF in E. coli for protein production

    Depositor

    Elias Lolis

    Insert

    MIF (MIF Human)
    

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cx36-GCaMP

    Plasmid

    #123604

    Purpose

    Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

    Depositor

    John O'Brien

    Article

    Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

    Insert

    Connexin 36-GCaMP3 fusion (Gjd2 Mouse)
    

    Tags

    GCaMP3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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