173,636 results

1. PRDX1A-c001

   Plasmid

   #175473

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.

   Depositor

   Opher Gileadi

   Insert

   PRDX1 (PRDX1 Human)
   

   Use

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBiFC-VC155

   Plasmid

   #22011

   Purpose

   Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   VC155
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Alanine 206 to Lysine

   Promoter

   Available since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. PhyB-mCherry-PEX3

   Plasmid

   #51572

   Purpose

   Constitutively express peroxisome localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   PEX3 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pD649-HAsp-TNFRSF12A-COMP5AP-AviTag-9xHis

   Plasmid

   #157052

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   TNFRSF12A (TNFRSF12A Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-fDIO-mCherry-WPREpA

   Plasmid

   #177328

   Purpose

   Control vector for Flp-dependent DREADD constructs

   Depositor

   Ulrik Gether

   Insert

   mCherry
   

   Use

   AAV; Flp-frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human Synapsin-1

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX

   Plasmid

   #202606

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P-P2A-mCherry-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBiFC-bJunVN173

   Plasmid

   #22012

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   bJunVN173 (Jun Aequorea Victoria, Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   c-Jun(257-338)-Venus(1-172)

   Promoter

   Available since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-15b-EK_C122S_His5

   Plasmid

   #49048

   Purpose

   Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

   Depositor

   Hans Brandstetter

   Article

   Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

   Insert

   Enteropeptidase, peptidase domain (TMPRSS15 Bovine)
   

   Use

   Tags

   5xHis

   Expression

   Bacterial

   Mutation

   P92R, E185D, C122S

   Promoter

   T7

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Integrin alpha L - mCFP

   Plasmid

   #8637

   Depositor

   Timothy Springer

   Article

   Kim et al Science 2003 Sep 19;301(5640):1720-5.

   Insert

   Integrin, alpha L (ITGAL Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2-nCas9n-nanos 3’UTR

    Plasmid

    #62542

    Purpose

    expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

    Depositor

    Antonio Giraldez

    Article

    Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

    Insert

    nanos 3' UTR (nanos3 Zebrafish)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    3' UTR only

    Promoter

    SP6

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tet-O-FUW-EGR3-P2A-mCherry

    Plasmid

    #203861

    Purpose

    Tet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    EGR3 (EGR3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid

    #177209

    Purpose

    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Yeast codon optimized

    Promoter

    Synthetic constitutive promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. dCas9-GCN5 core

    Plasmid

    #179551

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti6.2_mTagBFP2

    Plasmid

    #113725

    Purpose

    Expresses the fluorescent protein mTagBFP2 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Article

    Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

    Insert

    mTagBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS306-URA3-PHIS3-AqLumSynth –Sapphire

    Plasmid

    #116931

    Purpose

    Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    lumazine synthase
    

    Use

    Tags

    Sapphire

    Expression

    Yeast

    Mutation

    Promoter

    HIS3

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. TUBG1_pLX307

    Plasmid

    #98376

    Purpose

    Lentiviral expression of TUBG1

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    TUBG1 (TUBG1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-ER-mRFP

    Plasmid

    #231180

    Purpose

    Expresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, from a lentiviral vector

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    ER-mRFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL-p27UTR

    Plasmid

    #20881

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p27 3'UTR (Cdkn1b Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

19. Integrin-β6 I domain I270C

    Plasmid

    #159639

    Purpose

    Contains integrin beta 6 residues 108–352 with I270C mutation

    Depositor

    Timothy Springer

    Article

    Dong et al Nature. 2017 Feb 2;542(7639):55-59. doi:

    Insert

    Integrin beta 6 I domain I270C
    

    Use

    Tags

    6x His

    Expression

    Mammalian

    Mutation

    I270C

    Promoter

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-CRY2-mCherry

    Plasmid

    #58368

    Purpose

    Mamlian codon optimized CRY2

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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