We narrowed to 16,737 results for: Mpl;

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pEBB-II-I-GFP alpha isoform

Plasmid

#22146

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
L1_KanR-Ck1

Plasmid

#136123

Purpose

L1 in position 1 (next to LB in L2 construct), for KanR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpEF1a:nptII_3t35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCk1_spacer

Plasmid

#136071

Purpose

Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSR40

Plasmid

#69257

Purpose

plin-31::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

lin-31

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z20

Plasmid

#120063

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 10xSuntag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

10xSuntag

Use

Pcr template

Tags

10xSuntag

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-beta-hRLuc-V5-6xbsMS2-MALAT_X

Plasmid

#147924

Purpose

Mammalian Expression of 6xbsMS2-Malat

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

6xbsMS2-Malat (Malat1 Mouse)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I alpha

Plasmid

#22154

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
mApple-ERM

Plasmid

#217377

Purpose

ER marker

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

rat cb5 ERM

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-ELYS-emerin-EGFP-polyA

Plasmid

#59745

Purpose

Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

ELYS-emerin-EGFP

Tags

EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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Munc18cwt-pDEST27

Plasmid

#85626

Purpose

GST tagged Munc18c

Depositor

David James

Article

Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 20

Insert

Munc18c (Stxbp3 Mouse)

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-PAmCherry1_160-236-MCS

Plasmid

#60546

Purpose

Cloning PAmCherry1 C-terminal fragment (RC) for BiFC-PALM

Depositor

Xiaolin Nan

Article

Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

Insert

PAmCherry1 C-terminal fragment

Use

Cloning

Mutation

amino acids 160-236

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-PAmCherry1_1-159-MCS

Plasmid

#60545

Purpose

Cloning PAmCherry1 N-terminal fragment (RN) for BiFC-PALM

Depositor

Xiaolin Nan

Article

Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

Insert

PAmCherry1 N-terminal fragment

Use

Cloning

Mutation

amino acids 1-159

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-NPM NLS2D

Plasmid

#13285

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa150-aa155

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pMAL724

Plasmid

#169151

Purpose

Encodes OptoQ-AMP5

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 human TBC1D7 (19-293)

Plasmid

#132617

Purpose

expresses human TBC1D7 (19-293) in E. coli

Depositor

Wenqing Xu

Article

Qin et al J Biol Chem. 2016 Apr 15;291(16):8591-60

Insert

TBC1D7 (19-293)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z17

Plasmid

#120060

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mEos4b

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mEos4b

Use

Pcr template

Tags

mEos4b

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Integrin alpha5(1-623)-AHCys

Plasmid

#27301

Depositor

Timothy Springer

Article

Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

Insert

integrin alpha 5 (ITGA5 Human)

Expression

Mammalian

Mutation

alpha 5(1-623)+GG+TEV+GGEN+AHCys

Available Since

March 8, 2011

Availability

Academic Institutions and Nonprofits only
pMaCTag-P14

Plasmid

#120025

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeptune2.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeptune2.5

Use

Pcr template

Tags

mNeptune2.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET16b-Trx C35S

Plasmid

#36947

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin-1 C35S mutation (TXN Human)

Tags

His tag

Expression

Bacterial

Mutation

cys 35 to serine

Promoter

T7

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
pET21c-IF3mt

Plasmid

#31078

Depositor

Linda Spremulli

Article

Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

Insert

Mature form human mitochondrial translational initiation factor 3 (MTIF3 Human)

Tags

His

Expression

Bacterial

Mutation

Includes coding sequence for the mature form. Ami…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only