Addgene

We narrowed to 2,134 results for: mt

Showing: 961 - 990 of 2134 results

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pET15b-mLin28A-cHIS

Plasmid

#137998

Purpose

Lin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) cells

Depositor

Judy Lieberman

Article

O'Day et al J Biol Chem. 2015 Jul 17;290(29):17909-2

Insert

mouse Lin28A, CSD and ZnFs (residues 36–186)

Use

Tags

C-terminal His6 tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p37)

Plasmid

#135939

Purpose

Expresses AUF1p37 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2 and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p45)

Plasmid

#135942

Purpose

Expresses AUF1p45 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2 and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-6XPRE-GFP

Plasmid

#206171

Purpose

pMVP Gateway destination vector (3rd generation lentiviral vector) containing 6 consensus PGR binding sites upstream of a minimal 2YBTATA promoter

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

6X PRE-eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

2YBTATA

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag OSM

Plasmid

#12191

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

OSM (Ccm2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-TORCAR-NES

Plasmid

#175015

Purpose

Cytosol-targeted TORCAR.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

TORCAR-NES

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SpyCas9-AIDx

Plasmid

#135343

Purpose

Expression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) neo with Ywhag CDS

Plasmid

#61799

Purpose

pcDNA3.1(+) with full length mouse Ywhag CDS

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Ywhag CDS (Ywhag Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pTn7CdrA-gfpC

Plasmid

#111616

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(Mut3)-T0-T1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV Myc P57S IRES GFP

Plasmid

#18771

Depositor

Scott Lowe

Article

Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

Insert

myc P57S (MYC Human)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

P57S

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pSP64-hKCNE1

Plasmid

#53049

Purpose

Expresses beta subunit for IKs channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

Insert

Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

Promoter

SP6

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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ABEMax nSaCas9

Plasmid

#135364

Purpose

Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax nSaCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-AktAR2

Plasmid

#175013

Purpose

Plasma membrane-targeted AktAR2.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

Lyn-mCherry-Akt-STOPS

Use

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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PGRMC2-CD4d3+4-bio

Plasmid

#73112

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding PGRMC2 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

PGRMC2 (PGRMC2 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pGCaMP5-DHFR

Plasmid

#78706

Purpose

GCaMP5 under the Toxoplasma SAG1 5' UTR. Encodes a DHFR selectable marker.

Depositor

Sebastian Lourido

Article

Sidik et al J Biol Chem. 2016 Apr 29;291(18):9566-80

Inserts

GCaMP5
DHFR

Use

Tags

Expression

Mutation

Promoter

Toxoplasma SAG1 5' UTR

Available since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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C8orf55-CD4d3+4-bio

Plasmid

#73126

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding C8orf55 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

C8orf55 (THEM6 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Hmga2 tr

Plasmid

#14793

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Hmga2 truncated (Hmga2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

truncataed HMGA2. As in human tumors, Hmga2-tr la…

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV4-p100SS/AA (SS866/870AA)

Plasmid

#27555

Depositor

Shao-Cong Sun

Article

Xiao et al EMBO J. 2001 Dec 3. 20(23):6805-15.

Insert

p100 (NFKB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Serines 866 and 870 mutated to Alanines

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
CFP-OSM

Plasmid

#12190

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

OSM (Ccm2 Mouse)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
ama1CD4d3+4-bio

Plasmid

#36145

Purpose

EXPRESs plasmid for P. falciuparum merozoite surface proteins encoding P. falciparum ama1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ama1 (PF3D7_1133400 Plasmodium falciparum)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2012

Availability

Academic Institutions and Nonprofits only

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pShuttle-EGFP-CLASP2 340-1084

Plasmid

#24383

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1084) (CLASP2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

CLASP2 deletion mutant that retains MT lattice bi…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pHA843 - hrp-1p::hrp-1HsLCdeltaLC

Plasmid

#139200

Purpose

Expresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

stop at beginning of last exon

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CTA2-mNG211

Plasmid

#141167

Purpose

Expresses GST-SUMO tagged CTA2 Subunit with 11th Strand

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

SUMO-CTA2-mNG211

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE

Plasmid

#168108

Purpose

Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR

Depositor

Erich Jarvis

Article

Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

Type

Empty backbone

Use

CRISPR and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-CAG-F14F15S-BFP2-(mCherry)'

Plasmid

#115235

Purpose

Lentiviral transfer plasmid for expression of mTagBFP2 before Flp recombination and mCherry after it

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

mTagBFP2 and mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK-WW-CR-CT

Plasmid

#18069

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pET28a His6Flag2AUF1(p42)

Plasmid

#135941

Purpose

Expresses AUF1p42 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2 and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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GST-SF3B2 wild type aa 401-550 fragment

Plasmid

#67615

Purpose

bacterial expression of wild type GST-SF3B2 fragment (401-550)

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

splicing factor 3b subunit 2 (SF3B2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

fragment of amino acids 401-550

Promoter

tac

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pRVdG-4FLPo

Plasmid

#122050

Purpose

Genome plasmid for deletion-mutant rabies virus encoding Flpo recombinase

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

FLPo

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pJ4M_hnRNPA2_FL_P298L

Plasmid

#139111

Purpose

Express FL hnRNPA2 P298L with a C-terminal MBP tag

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

Expression

Bacterial

Mutation

P298L

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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