We narrowed to 158,093 results for: Des

1. pSEVA421-Cas9tr

   Plasmid

   #138709

   Purpose

   Plasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenes

   Depositor

   Víctor de Lorenzo

   Article

   Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

   Insert

   tracrRNA-Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2 GFP-GSK3mut-MAPK

   Plasmid

   #29690

   Purpose

   GFP reporter protein fused to three mutated GSK3 phosphorylation sites

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
   

   Use

   Tags

   Flag and Flag-EGFP

   Expression

   Mammalian

   Mutation

   recognition sites only, and E3 ligase site, GSK3 …

   Promoter

   SP6

   Available Since

   Jan. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV-LIN28A

   Plasmid

   #117057

   Purpose

   Overexpression of mouse LIN28A

   Depositor

   Michele De Palma

   Article

   Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

   Insert

   LIN28A (Lin28a Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hPGK promoter

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLPC hRap1-FL

   Plasmid

   #12542

   Depositor

   Titia de Lange

   Article

   Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

   Insert

   Repressor Activator Protein 1 (TERF2IP Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

5. VPS13D^mScarlet

   Plasmid

   #174110

   Purpose

   The insert is a codon optimized DNA sequence encoding VPS13D with an internal mScarlet protein.

   Depositor

   Pietro De Camilli

   Article

   Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   Codon optimized VPS13D
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   With an internal mScarlet tag in aminoacid positi…

   Promoter

   CMV

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCD5-H3TX12-CO-GCN4-TEV-sfGFP-TS

   Plasmid

   #158739

   Purpose

   Expresses influenza A virus trimeric hemagglutinin A/H3/TX/12 fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

   Insert

   Hemagglutinin A/TX/50/12 H3
   

   Use

   Tags

   GCN4-TEV-sfGFP-TwinStrep

   Expression

   Mammalian

   Mutation

   G186V S219F

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. ER-mCherry-eMagA

   Plasmid

   #162248

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   CytP450 ER targeting sequencel-mCherry-eMagA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   beta-actin promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNQL004-SOX2-FKBPV-HA2-P2A-mCherry targeting construct

   Plasmid

   #175552

   Purpose

   Targeting vector to introduce an FKBPV-HA2-P2A-mCherry cassette at the mouse SOX2 locus. Degradation Tag (dTAG) system.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   SOX2 (Sox2 Mouse)
   

   Use

   Mouse Targeting

   Tags

   FKBPV-HA2-P2A-mCherry

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. eMagA-mCherry-Mito

   Plasmid

   #162251

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   eMagA-mCherry-OMP25
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   beta-actin promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPC-NMYC TRF2

    Plasmid

    #16066

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF2 (TERF2 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    aa2-500, inserted alanin at aa434

    Promoter

    Available Since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-PIPK1 gamma 87

    Plasmid

    #22300

    Depositor

    Pietro De Camilli

    Article

    Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

    Insert

    human phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX335-NQL002-WAPL-sgRNA1

    Plasmid

    #175550

    Purpose

    For transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL003-WAPL-sgRNA2 targeting construct.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nickase and sgRNA against mouse WAPL STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX335-NQL003-WAPL-sgRNA2

    Plasmid

    #175551

    Purpose

    For transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL002-WAPL-sgRNA1 targeting construct.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nickase and sgRNA against mouse WAPL STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWZL Hygro-NMYC TRF2 deltaB deltaM

    Plasmid

    #16355

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF2 (TERF2 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

    Promoter

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pNQL006-NANOG-FKBPV-HA2-P2A-eGFP targeting construct

    Plasmid

    #175554

    Purpose

    Targeting vector to introduce an FKBPV-HA2-P2A-eGFP cassette at the mouse NANOG locus. Degradation Tag (dTAG) system.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    NANOG
    

    Use

    Mouse Targeting

    Tags

    FKBPV-HA2-P2A-eGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3xFLAG CMV14 / BRAF-D594G

    Plasmid

    #131719

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D594G

    Promoter

    CMV

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-Dynamin 2 K44A

    Plasmid

    #22301

    Depositor

    Pietro De Camilli

    Article

    Ochoa et al J Cell Biol. 2000 Jul 24. 150(2):377-89.

    Insert

    Dynamin 2 K44A (Dnm2 Rat)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    K44A

    Promoter

    Available Since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

18. HA-mDyn2 pcDNA3

    Plasmid

    #36264

    Depositor

    Pietro De Camilli

    Article

    Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

    Insert

    Dynamin 2 (Dnm2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 20, 2012

    Availability

    Academic Institutions and Nonprofits only

19. VPS13C^Halo-△(ATG2C-PH)

    Plasmid

    #232867

    Purpose

    Expresses VPS13C-△(ATG2C-PH) in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

    Insert

    VPS13C-△(ATG2C-PH) (VPS13C Human)
    

    Use

    Tags

    internal Halo

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-PH(VPS13C)

    Plasmid

    #232869

    Purpose

    Expresses the PH domain of VPS13C in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

    Insert

    the PH domain of VPS13C (VPS13C Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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