We narrowed to 24,827 results for: Spr

1. lentiCRISPRv2-TTF-1-gRNA

   Plasmid

   #101857

   Purpose

   Introducing a gRNA targeting human TTF-1 (NKX2-1) into cells

   Depositor

   David Mu

   Article

   Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

   Insert

   gRNA targeting TTF-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. B52 + SPRTN sgSTOP

   Plasmid

   #100709

   Purpose

   B52 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting SPRTN (cloned using BbsI)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoters

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. R777-E329 Hs.SPRY2

   Plasmid

   #70613

   Purpose

   Gateway ORF clone of human SPRY2 [NM_005842.2] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   SPRY2 (SPRY2 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti CRISPR V2 sgMTHFD1L_1

   Plasmid

   #106316

   Purpose

   Express Cas9 and sgRNA targeting MTHFD1L

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting MTHFD1L
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. B270 + SPRTN sgSTOP

   Plasmid

   #100718

   Purpose

   B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting SPRTN (cloned using BbsI)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoters

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pISV-CRISPR/Cas9PGmUbi

   Plasmid

   #209189

   Purpose

   Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection

   Depositor

   Christian Staehelin

   Inserts

   Cas9
   DsRed(deltaB)
   

   Tags

   NLS

   Expression

   Plant

   Mutation

   missing BsaI site and plant-codon optimized with …

   Promoter

   CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti CRISPR sgMTHFD2_1

   Plasmid

   #106313

   Purpose

   Express Cas9 and sgRNA targeting MTHFD2

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting MTHFD2 (MTHFD2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWS5394-CRISPRai-MET17

   Plasmid

   #182833

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - MET17 marker

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL0426 (CRISPR with spc3)

   Plasmid

   #130642

   Purpose

   Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   CRISPR(spacer-3)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. R777-E333 Hs.SPRY4

    Plasmid

    #70618

    Purpose

    Gateway ORF clone of human SPRY4 [NM_001127496.1] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    SPRY4 (SPRY4 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPR - EGFP sgRNA 6

    Plasmid

    #51765

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 6
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPRv2-Blast-mU6

    Plasmid

    #206806

    Purpose

    pLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassette

    Depositor

    Jolanda Van Leeuwen

    Article

    Unlu et al Genome Med. 2023 Oct 12;15(1):78. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZR064_CROPseq-sgCD4-CRISPRi

    Plasmid

    #180270

    Purpose

    Lentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRi

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    CD4 sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWS5392-CRISPRai-HIS3

    Plasmid

    #182831

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - HIS3 marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWS5393-CRISPRai-TRP1

    Plasmid

    #182832

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - TRP1 marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWS5395-CRISPRai-LYS2

    Plasmid

    #182834

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - LYS2 marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWS5391-CRISPRai-LEU2

    Plasmid

    #182830

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - LEU2 marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-CRISPR-v2-gYap-1

    Plasmid

    #166481

    Purpose

    Expresses spCas9 and a gRNA targeting mouse Yap

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    gRNA for mouse Yap (Yap1 Mouse)
    

    Use

    Lentiviral

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiCRISPRv2-sgLmnb2-HepmCherry

    Plasmid

    #192830

    Purpose

    Expresses sgRNA targeting Lmnb2 and mCherry from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 for sgRNA and hepatocyte-specific promoter (HS…

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCRISPRv2-Stuffer-HepmTurquoise2

    Plasmid

    #192826

    Purpose

    Contains stuffer sequence into which sgRNAs can be cloned and expresses mTurquoise2 from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mTurquoise2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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