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pCRISPR_DMS3g24

Plasmid

#89244

Purpose

Plasmid encodes a synthetic CRISPR containing 7 copies of a single spacer, which targets a region (gene 24) in the genome of bacteriophage DMS3

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Insert

CRISPR

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-CmCHS-CmCHI

Plasmid

#73395

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
CmCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Pc4CL-CmCHS-MsCHI

Plasmid

#73398

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
CmCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-PhCHS-CmCHI

Plasmid

#73393

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Vv4CL-PhCHS-CmCHI

Plasmid

#73401

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pEJS507-pCDest2-noAcr-mTagBFP2-IRES

Plasmid

#85748

Purpose

Expresses mTagBFP2 only; used as a control

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6-Vv4CL-CmCHS-MsCHI

Plasmid

#73402

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
CmCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Pc4CL-PhCHS-CmCHI

Plasmid

#73397

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pEJS427-pCSDest2-AcrE2

Plasmid

#85677

Purpose

Mammalian expression of anti-CRISPR protein from Type I-E

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type I-E anti-CRISPR

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6-Vv4CL-CmCHS-CmCHI

Plasmid

#73403

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
CmCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Pc4CL-CmCHS-CmCHI

Plasmid

#73399

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
CmCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBT281

Plasmid

#122611

Purpose

express evoAPOBEC1-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT375

Plasmid

#125616

Purpose

Expresses evoAPOBEC1-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max-NG

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-Flavidin

Plasmid

#89881

Purpose

Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling

Depositor

Mark Howarth

Article

Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

Insert

Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag

Tags

6 glutamate tag (C terminal on insert)

Expression

Bacterial

Mutation

K80R, K121R, K132R, K134R

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

Plasmid

#73404

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pEJS469-pLK.O1-SpySgRNA/DTS13-Telomere

Plasmid

#85715

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

telomere sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Stat1 alpha Y701F Flag pRc/CMV

Plasmid

#8702

Depositor

Jim Darnell

Article

Wen et al Cell 1995 Jul 28;82(2):241-50.

Insert

STAT1 alpha Y701F (STAT1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Y701F

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
Stat1 alpha S727A pRc/CMV

Plasmid

#8700

Depositor

Jim Darnell

Article

Wen et al Cell 1995 Jul 28;82(2):241-50.

Insert

STAT1 alpha S727A (STAT1 Human)

Expression

Mammalian

Mutation

S727A

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
Stat1 alpha Y701F pRc/CMV

Plasmid

#8701

Depositor

Jim Darnell

Article

Wen et al Cell 1995 Jul 28;82(2):241-50.

Insert

STAT1 alpha Y701F (STAT1 Human)

Expression

Mammalian

Mutation

Y701F

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomere

Plasmid

#85714

Purpose

Telomere-targeting Nme sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

telomere sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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