We narrowed to 1,853 results for: rigi

1. Nup54-mEGFP494(1)

   Plasmid

   #163427

   Purpose

   Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of the mEGFP

   Depositor

   Sanford Simon

   Insert

   Nup54 (NUP54 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pREC1010C-Eco1

   Plasmid

   #240690

   Purpose

   RSF1010 origin of replication plasmid containing Eco1 recombitron with a SapI flanked stuffer in the ncRNA expressed by J23115 constitutive promoter

   Depositor

   Seth Shipman

   Insert

   Eco1 RT, Eco1 ncRNA, CspRecT and EcSSB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR182

   Plasmid

   #240642

   Purpose

   Template for in vitro transcription of AmNeonGreen mRNA using SP6 polymerase.

   Depositor

   John Pringle

   Insert

   AmNeonGreen
   

   Use

   Vertebrate gene expression and general in vitro t…

   Tags

   Expression

   Mutation

   Codon-optimized for Exaiptasia diaphana, amino ac…

   Promoter

   SP6

   Available Since

   Aug. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pREC1010-Abe1

   Plasmid

   #240683

   Purpose

   RSF1010 origin of replication plasmid containing Abe1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter

   Depositor

   Seth Shipman

   Insert

   Abe1 RT, Abe1 ncRNA, CspRecT and EcSSB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available Since

   Aug. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Nup54-mEGFP494(2)

   Plasmid

   #163428

   Purpose

   Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of the mEGFP

   Depositor

   Sanford Simon

   Insert

   Nup54 (NUP54 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pR6K-Donor(PmcCAST)_KanR

   Plasmid

   #224928

   Purpose

   Transposition assay

   Depositor

   Leifu Chang

   Article

   Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

   Insert

   LE_cargo_RE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. p426TEF-LptTPS-A

   Plasmid

   #89468

   Purpose

   Expression of prespatane synthase LptTPS-A in S. cerevisiae for sesquiterpene production

   Depositor

   Jing-Ke Weng

   Insert

   LptTPS-A
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   Promoter

   pBR322 origin of replication for propagation in E…

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pxCas9CR4

   Plasmid

   #111656

   Purpose

   PCas9CR4 with the xCas9 mutations allowing NG PAM sites

   Depositor

   Christopher Reisch

   Insert

   TetR and xCas9
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   xCas9 mutations

   Promoter

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-HLA-B15-His

   Plasmid

   #104837

   Purpose

   HLA-B150101 cloned as a His tag

   Depositor

   Sébastien Walchli

   Insert

   major histocompatibility complex, class I, B (HLA-B Human)
   

   Use

   Entry vector for gateway system

   Tags

   his

   Expression

   Mutation

   2 differences compared to original B15: L2->R …

   Promoter

   Available Since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pREC1010-opEbu1

    Plasmid

    #240702

    Purpose

    RSF1010 origin of replication plasmid containing Ebu1 recombitron with extended a1 a2 regions with a donor in the ncRNA targeting lacZ locus in Citrobacter freundii ATCC 8090 expressed by Pm promoter

    Depositor

    Seth Shipman

    Insert

    Ebu1 RT, Ebu1 ncRNA, CspRecT and EcSSB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Extended a1 a2 regions

    Promoter

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMP71-HLA-B15-His

    Plasmid

    #104848

    Purpose

    HLA-B150101 cloned as a His tag

    Depositor

    Sébastien Walchli

    Insert

    major histocompatibility complex, class I, B (HLA-B Human)
    

    Use

    Retroviral

    Tags

    his

    Expression

    Mammalian

    Mutation

    2 differences compared to original B15: L2->R …

    Promoter

    LTR

    Available Since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. HOXD4-mam.exp.

    Plasmid

    #21003

    Depositor

    Corey Largman

    Insert

    HOXD4 (Hoxd4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pST39

    Plasmid

    #64009

    Purpose

    original polycistronic cloning vector for plasmid suite (generation I)

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCPCbLCVB.002

    Plasmid

    #22787

    Depositor

    Niki Robertson

    Article

    Turnage et al Plant J. 2002 Apr . 30(1):107-14.

    Insert

    B DNA
    

    Use

    RNAi; Arabidopsis vigs vector

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available Since

    July 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMTCbLCVA::CH42

    Plasmid

    #22786

    Depositor

    Niki Robertson

    Article

    Turnage et al Plant J. 2002 Apr . 30(1):107-14.

    Insert

    A DNA containing CHLI fragment
    

    Use

    RNAi; Arabidopsis vigs vector

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available Since

    July 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. psubCAG-WPRE-hVEGF-D-Strept

    Plasmid

    #119227

    Purpose

    Plasmid encodes Strep-tagged full-length (unprocessed) VEGF-D of human origin to be used for stimulation of lymphangiogenesis in mice in vivo.

    Depositor

    Kari Alitalo

    Insert

    VEGFD (VEGFD Human)
    

    Use

    AAV

    Tags

    Strept

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE-60NA-mScarlet-I

    Plasmid

    #160462

    Purpose

    Encodes the original mScarlet-I

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. HOXD4-FS-mam.exp.

    Plasmid

    #21004

    Depositor

    Corey Largman

    Insert

    HOXD4 (Hoxd4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Frame shift mutant--No DNA binding

    Promoter

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

19. lentiCRISPR v2-Blast

    Plasmid

    #83480

    Purpose

    Mammalian expression of Cas9 and sgRNA scaffold

    Depositor

    Mohan Babu

    Insert

    Blasticidin S deaminase
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    EF-1α

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMU131

    Plasmid

    #66811

    Purpose

    Shuttle vector with gram-positive origin of replication isolated from a native plasmid of Thermoanaerobacterium saccharolyticum B6A-RI

    Depositor

    Lee Lynd

    Article

    Shaw et al Appl Environ Microbiol. 2010 Jul;76(14):

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge