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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,008 results for: rigi

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Showing: 641 - 660 of 2008 results

  1. pAGE3.0

    Plasmid
    #165985
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Nm/Kan resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    neomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Available Since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    165985-plasmid-map-sequence-id-375864

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  3. pCW-ND-Cdt1ΔR198A/R210A-HA-Puro

    Plasmid
    #193769
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding (R198A/R210A), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1ΔR198A/R210A (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193769-plasmid-map-sequence-id-380211

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  4. pCW-ND-Cdt1Δ499-546-HA-Puro

    Plasmid
    #193768
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1Δ499-546 (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193768-plasmid-map-sequence-id-380214

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  5. pCW-ND-Cdt1-mCherry-Puro

    Plasmid
    #193766
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1-mCherry (CDT1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193766-plasmid-map-sequence-id-380221

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  10. Escherichia coli DH10B-MOB

    Bacterial Strain
    #229395
    Purpose
    Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)
    Depositor
    Michael Springer
    Article
    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1
    Bacterial Resistance
    60 ug/mL of DAP
    Species
    Escherichia coli
    Available Since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only

  11. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid
    #193139
    Purpose
    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    Use
    Lentiviral
    Mutation
    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…
    Promoter
    EF1a
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193139-plasmid-map-sequence-id-380234

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  12. pStABK1

    Plasmid
    #193729
    Purpose
    Start-Stop assembly level 1 vector, BBR1 origin, kanR
    Depositor
    Martin Buck
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193729-plasmid-map-sequence-id-381921

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  13. VfAU1-LOV_136

    Plasmid
    #63632
    Purpose
    VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by a restriction site
    Depositor
    Harald Janovjak
    Insert
    VfAU1-LOV
    Expression
    Mammalian
    Available Since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63632-plasmid-map-sequence-id-108793

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  14. pfNL43-dE-EGFP

    Plasmid
    #36865
    Depositor
    Mario Chin
    Insert
    fNL43-dE-EGFP
    Use
    Lentiviral and Retroviral
    Expression
    Mammalian
    Mutation
    inserted EGFP after 6350 nucleotide of original N…
    Available Since
    Aug. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    36865-plasmid-map-sequence-id-46078

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  15. pLSU1/o35S[]:MCS:NosT

    Plasmid
    #212163
    Purpose
    This binary vector has an empty backbone where you can insert a gene into a cassette with a strong promoter (original 35sCAMV)
    Depositor
    Wayne Curtis
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212163-plasmid-map-sequence-id-422668

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  16. pRAC01A

    Plasmid
    #193730
    Purpose
    Expresses deactivated-Cas9 (J23101) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion
    Depositor
    Martin Buck
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193730-plasmid-map-sequence-id-381916

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  17. pRAC07A

    Plasmid
    #193733
    Purpose
    Expresses deactivated-Cas9 (J23107) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion
    Depositor
    Martin Buck
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193733-plasmid-map-sequence-id-381918

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  18. MYR-VfAU1-LOV_135

    Plasmid
    #63631
    Purpose
    Expresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by a MYR signal and a restriction site
    Depositor
    Harald Janovjak
    Insert
    MYR-VfAU1-LOV
    Expression
    Mammalian
    Available Since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63631-plasmid-map-sequence-id-108771

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  19. MSCV-EcoR1-IRES-GFP

    Plasmid
    #66956
    Purpose
    original retroviral vector with one single EcoR1 cloning site
    Depositor
    Martine Roussel
    Type
    Empty backbone
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    MSCV
    Available Since
    Sept. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. (P2)RBSS7

    Plasmid
    #251372
    Purpose
    RBS originally used in pDF-lac [74]
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S7
    Expression
    Bacterial
    Available Since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    251372-plasmid-map-sequence-id-503741

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Showing: 641 - 660 of 2008 results