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pMSCVpuro-Venus

Plasmid

#96940

Purpose

Retroviral plasmid containing Venus for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Venus Fluorescent Protein

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-6His-AcpS

Plasmid

#145376

Purpose

Expression of E. coli AcpS

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

AcpS (acpP E. coli)

Tags

6-His

Expression

Bacterial

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pXT128_TwinStrep-SUMO-ShCas12k

Plasmid

#135524

Purpose

Protein expression construct for ShCast12k

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShCas12k

Tags

TwinStrep-SUMO

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCSC-SP-PW-NepX (aka: pBOB-NEPX)

Plasmid

#12340

Depositor

Inder Verma

Article

Marr et al J Mol Neurosci. 2004 . 22(1-2):5-11.

Insert

NEP-X (MME Human)

Use

Lentiviral

Expression

Mammalian

Mutation

E585V (inactive mutant)

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pGL0_3 [RSF1010 ORI]

Plasmid

#198920

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RSF1010 ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISYmu1_reRNA

Plasmid

#205358

Purpose

Bacterial expression of ISYmu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISYmu1 TnpB

Expression

Bacterial

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pET-15b-UbcH5b/2419

Plasmid

#11475

Depositor

Allan Weissman

Article

Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

Insert

UbcH5b (UBE2D2 Human)

Expression

Bacterial

Mutation

n/a

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pCiVSP-SD64TF

Plasmid

#80332

Purpose

Expresses Ciona VSP in Xenopus Oocyte

Depositor

Yasushi Okamura

Article

Murata et al Nature. 2005 Jun 30;435(7046):1239-43. E

Insert

Ciona intestinalis voltage-sensing phosphatase (vsp Ciona intestinalis)

Use

Xenopus oocyte expression

Promoter

SP6

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2cpV

Plasmid

#37471

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D2cpv second generation cameleon (calcium sensor)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pUA

Plasmid

#58372

Purpose

UAS vector to express gene of interest in Drosophila

Depositor

Chun Han, Yuh-Nung Jan

Article

Han et al Neuron. 2014 Feb 5;81(3):544-60. doi: 10

Type

Empty backbone

Expression

Insect

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-PRKAA1

Plasmid

#30305

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α1 (Prkaa1 Rat)

Tags

GFP

Expression

Mammalian

Available Since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

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DS-SPcas

Plasmid

#48645

Purpose

Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
tracrRNA precursor

Use

CRISPR

Promoter

proC and tracrRNA promoter

Available Since

Dec. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pPSint

Plasmid

#149421

Purpose

Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the IV2 intron in the GFP reporter gene.

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Type

Empty backbone

Expression

Plant

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pTrcAgBIS (CO)

Plasmid

#35153

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

Bisabolene Synthase

Use

Synthetic Biology

Expression

Bacterial

Promoter

pTrc

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pGL3-rev-erbα

Plasmid

#186823

Purpose

Fluorescent reporter for rev-erbα expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

rev-erbα promoter

Use

Luciferase

Promoter

rev-erbα promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISDge10_reRNA

Plasmid

#205340

Purpose

Bacterial expression of ISDge10 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISDge10 TnpB

Expression

Bacterial

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Alpha-synuclein

Plasmid

#181715

Purpose

Galactose inducible expression of Alpha-synuclein

Depositor

Alejandro Chavez

Insert

Alpha-synuclein (SNCA Human)

Expression

Yeast

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEE083

Plasmid

#149279

Purpose

T-DNA encoding TRV2 complete genome with MCS

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Type

Empty backbone

Expression

Plant

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pHC79

Plasmid

#167551

Purpose

Backbone plasmid to pPM1001, pPM1002, pPM1003, pPM2212 and pPM2213

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Type

Empty backbone

Use

Cosmid

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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LRT2B-AdGO2

Plasmid

#136911

Purpose

Lentivirus expressing AdGO2 sgRNA

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

sgAdGO2

Use

Lentiviral

Mutation

WT

Promoter

hU6

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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