We narrowed to 13,831 results for: sequence
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Plasmid#229984PurposeLIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.DepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only
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pPLP2H-mCf
Plasmid#229985PurposeLIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.DepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP3H-mCf
Plasmid#229987PurposeLIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.DepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
MDC1 KO sgRNA
Plasmid#207103PurposepX330 based plasmid for expression of Cas9 and the GGTGTAACGTGGAGCCAGTA sgRNA to target the MDC1 coding sequence.DepositorInsertGGTGTAACGTGGAGCCAGTA
ExpressionMammalianPromoterCMV and U6Available SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMM710
Plasmid#68893PurposeIPTG-inducible CRISPRi vector targeting nonsense sequence (NS), pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMH220
Plasmid#122836PurposedCas9 with GreenGate B-C flanking sequencesDepositorInsertNuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
UseGolden gate compatible cloning vectorMutationContains D10A and H840A mutationsAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
A1-20B1 (P001)
Plasmid#161820PurposeThis plasmid carries Yn situ preamplifier A1-20B1 sequence.DepositorInsertA1-20B1
UseSynthetic BiologyAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBYK01
Plasmid#138656PurposephiOZJ integrating Streptomyces expression vector lacking RDFDepositorTypeEmpty backboneExpressionBacterialPromoterPermE* variant sequence from pDA1652Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMD5
Plasmid#138652PurposephiBT1 integrating Streptomyces expression vectorDepositorTypeEmpty backboneExpressionBacterialPromoterPermE* variant sequence from pDA1652Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-Flp1)
Plasmid#194770PurposeTo insert T2A-Flp1 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Flp1 transgene.DepositorInsertT2A-Flp1
UseCRISPRExpressionInsectAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet28a-ClbB-NRPS-NHis
Plasmid#49217PurposeNRPS module of ClbB containing CAT domains with N-terminal 6His tagDepositorInsertClbB-NRPS
Tags6x His tag and T7 tagExpressionBacterialMutationcontains aa4-1080 of reference sequence NP_754362…PromoterT7Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQE30-TEV-pro-conA
Plasmid#159527PurposeExpresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.DepositorArticleInsertCanavalia ensiformis pro-concanavalin A
TagsSix-Histidine tag and tobacco etch virus (TEV) pr…ExpressionBacterialPromoterT5Available SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S
Plasmid#211839PurposePlant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.DepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationR181A R185APromoterUBIQUITIN10Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmNFM
Plasmid#83126PurposeMouse neurofilament protein M cDNA mammalian expression constructDepositorAvailable SinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMD13
Plasmid#138653PurposephiC31 integrating Streptomyces expression vectorDepositorTypeEmpty backboneExpressionBacterialPromoterPermE* variant sequence from pDA1652Available SinceJune 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-SUMO-TAG35-sfGFP
Plasmid#212120PurposeFluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-TAG35-sfGFP
M. alvus Pyl-tRNA
TagsHis6 and SUMO-TAG35ExpressionBacterialMutationE35TAG (within SUMO encoding sequence)PromoteraraC and lppAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP
Plasmid#112631PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral RGDSP integrin-binding sequencePromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-PICK1-rescue
Plasmid#72573PurposeExpresses myc-PICK1 shRNA resistant cDNADepositorInsertPICK1 (Pick1 Rat)
TagsMycExpressionMammalianMutation5 silent mutations around shRNA recognition seque…PromoterCMVAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -