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pDONR221_SLC12A6

Plasmid

#131956

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC12A6 (SLC12A6 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pME-Luciferase-P2A-H2A-mCherry (JDW 926)

Plasmid

#224488

Purpose

Gateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Use

Luciferase

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC5A9

Plasmid

#132230

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC5A9 (SLC5A9 Human)

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC25A47

Plasmid

#131983

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A47 (SLC25A47 Human)

Expression

Mammalian

Available Since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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p2R3a-VenusYFP-3AT

Plasmid

#71269

Purpose

Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

VenusYFP

Use

Gateway Cloning

Tags

4xGly linker and T3A pea Pisum sativum ribulose-1…

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC17A8

Plasmid

#131961

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC17A8 (SLC17A8 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SV2B

Plasmid

#132281

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SV2B (SV2B Human)

Expression

Mammalian

Available Since

May 19, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pT3EF5a-PIK3CA H1047R

Plasmid

#180034

Purpose

Entry vector of PIK3CA H1047R for gateway cloning.

Depositor

Xin Chen

Article

Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Gateway Cloning

Tags

HA tag

Expression

Mammalian

Mutation

H1047R

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 S_nostop

Plasmid

#152988

Purpose

Gateway-compatible Entry vector, with insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR1a-mCherry-STIM1

Plasmid

#114176

Purpose

Gateway entry clone containing mCherry-STIM1

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

STIM1 (STIM1 Human)

Use

Gateway Cloning

Tags

mCherry (inserted after signal peptide)

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC39A6

Plasmid

#132153

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC39A6 (SLC39A6 Human)

Expression

Mammalian

Available Since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV_GFAP_HyPer7_mito

Plasmid

#238966

Purpose

Mammalian astrocytes HyPer7 expression targeted to the mitochondrial matrix

Depositor

Dmitry Bilan

Article

Kotova et al Free Radic Biol Med. 2023 Nov 1;208:153-

Insert

HyPer7

Use

AAV

Tags

3x Cytochrome C Oxidase Subunit VIII presequense

Expression

Mammalian

Promoter

GFAP

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221_TMEM104

Plasmid

#132262

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

TMEM104 (TMEM104 Human)

Expression

Mammalian

Available Since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC46A2

Plasmid

#132203

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC46A2 (SLC46A2 Human)

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pEN_TT 3XFLAG-wtGATA6-3XAU1

Plasmid

#72612

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Gateway Cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Promoter

TRE tight promoter

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC9A6

Plasmid

#132175

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC9A6 (SLC9A6 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC25A30_STOP

Plasmid

#161218

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A30 (SLC25A30 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC17A6

Plasmid

#131948

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC17A6 (SLC17A6 Human)

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC5A8

Plasmid

#132218

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC5A8 (SLC5A8 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC9B2

Plasmid

#132235

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC9B2 (NHEDC2 Human)

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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