164,060 results

1. SPLICSFAST-ER-PO-P2A

   Plasmid

   #214894

   Purpose

   Expresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-ER-PO-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPLICS Mt-ER- PM Short P2A

   Plasmid

   #164110

   Purpose

   Detect the short Plasma membrane-mitochondria-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP and YFP PM- Mt-ER Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSFDuet-1(nsp8-nsp7)(nsp12)

   Plasmid

   #165451

   Purpose

   Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

   Depositor

   Marc Delarue

   Article

   Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

   Inserts

   nsp12
   nsp8
   nsp7
   

   Use

   Tags

   14Histidine-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_816

   Plasmid

   #201157

   Purpose

   EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   5x-ALFA tag-dEnAsCas12a-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D908A

   Promoter

   EFS

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCH61 (multiAsCas12a-KRAB piggyBac)

   Plasmid

   #217332

   Purpose

   piggyBac expression of multiAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   multiAsCas12a
   

   Use

   CRISPR

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   R1226A/E174R/S542R/K548R

   Promoter

   CAG

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet-1_DNAseI

   Plasmid

   #182408

   Purpose

   Bacterial expression of DNaseI

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   DNase I (DNASE1 Bovine)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PiggyBac PlayBack

   Plasmid

   #203312

   Purpose

   To all for PiggyBac gene insertion into Genome

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-3XMyc-EGFP-OMP25

   Plasmid

   #83355

   Purpose

   For tagging mitochondria with Myc epitopes (Control-MITO construct)

   Depositor

   David Sabatini

   Article

   Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

   Insert

   3XMyc-EGFP-OMP25
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Mfn1

   Plasmid

   #141154

   Purpose

   Mammalian expression and labeling mitofusin 1

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   Mitofusin 1 (MFN1 Human)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDF-5xT7-TtCsm

    Plasmid

    #128572

    Purpose

    For expression of T. thermophilus Csm in E. coli (Csm1-5)

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

    Inserts

    Csm1
    Csm2, Csm3, Csm4, Csm5
    

    Use

    Tags

    HRV 3C protease cleavage site and 10xHis tag on C…

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli and Cod…

    Promoter

    T7 promoter, lac operator

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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