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Halo-ATG9A HRD

Plasmid

#203222

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG9A locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human ATG9 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-CCSP:GFP

Plasmid

#68487

Purpose

Retrovirus expressing GFP driven by CCSP promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

CCSP and LTR

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Tornado-RNA sensor for MALAT-1

Plasmid

#185406

Purpose

circular RNA-scaffolded sensing modules for MALAT-1

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

circular RNA-scaffolded sensing modules

Expression

Mammalian

Mutation

WT

Promoter

U6

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CWB-yellow pre-eGRASP(p30)

Plasmid

#111587

Purpose

An AAV vector that expresses yellow pre-eGRASP(p30) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

yellow pre-eGRASP(p30)

Use

AAV

Promoter

CaMKIIa

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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JC201: pMVP/Ad/5-35-DEST

Plasmid

#121847

Purpose

pMVP destination vector, empty 5/35 fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Map3k1

Plasmid

#29581

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Map3k1 (MAP3K1 Human)

Tags

GST, HIS6, and TEV

Expression

Bacterial

Mutation

Constitutively Active. Contains catalytic domain …

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
Bxb1-GA_CAG-EGFP

Plasmid

#229799

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of EGFP

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAPtag5-IgK-NCAN-Full length

Plasmid

#219812

Purpose

Expresses His-tagged NCAN-Full length to produce recombinant protein

Depositor

Cagla Eroglu

Article

Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

Insert

IgK (Igk Mouse)

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt7

Plasmid

#184508

Purpose

Mammalian expression of Synaptotagmin 7 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 7 (Syt7 Mouse)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA Donor_hEF1a

Plasmid

#229779

Purpose

Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-CMV:GFP

Plasmid

#68485

Purpose

Retrovirus expressing GFP driven by CMV promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

CMV and LTR

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pHes1-DN-GFP (CC#110)

Plasmid

#15134

Depositor

Connie Cepko

Article

Furukawa et al Neuron. 2000 May . 26(2):383-94.

Insert

Hes1 (Hes1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

E43A, K44A, R47A

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
pTnos (GB0037)

Plasmid

#68188

Purpose

Provides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Nos terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pEBB-3XMyc-TRAF2

Plasmid

#44104

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pL1P3 ZmUbiP:GRF-GIF:NosT

Plasmid

#198047

Purpose

MoClo Golden Gate Level 1 Position 3. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

Depositor

Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

Insert

ZmUbiP::TaGRF4-GIF1::NosT

Use

Synthetic Biology

Promoter

Zea mays (maize) ubiquitin promoter

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pIVT-Bxb1

Plasmid

#232746

Purpose

IVT template for making modRNA encoding Bxb1

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEvoltac-EcW-TGA-h14

Plasmid

#218764

Purpose

encodes EcWRS-h14 and EcW-tRNA-TGA

Depositor

Abhishek Chatterjee

Article

Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

Insert

EcWRS-h14

Expression

Bacterial

Mutation

S8A, V144G, V146C

Promoter

tacI

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCW-LIC-N8C_Fab3b

Plasmid

#203685

Purpose

Expresses N8C_Fab3b

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab3b

Expression

Bacterial

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-KB1691-Nluc

Plasmid

#204324

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

KB1691-Nluc

Expression

Mammalian

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV Puro-pGK:GFP

Plasmid

#68486

Purpose

Retrovirus expressing GFP driven by pGK promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR and pGK

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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