166,461 results

1. pCAG_mRuby2_smFP FLAG

   Plasmid

   #59760

   Purpose

   encodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   mRuby2_smFP_FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-HA-ER D538G

   Plasmid

   #49500

   Purpose

   Expresses HA-tagged ER D538G mutant in mammalian cells

   Depositor

   Sarat Chandarlapaty

   Article

   Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

   Insert

   ESR1 (ESR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Aspartic acid 538 to Glycine

   Promoter

   CMV

   Available Since

   Dec. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-hSyn-EGFP

   Plasmid

   #50465

   Purpose

   EGFP under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, and AAV9 trial size

   Insert

   EGFP
   

   Use

   AAV

   Tags

   N/A

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available Since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. TEVSH

   Plasmid

   #125194

   Purpose

   His-tagged TEV protease with solubility increasing mutations

   Depositor

   Helena Berglund

   Article

   van den Berg et al J Biotechnol. 2006 Feb 10;121(3):291-8.

   Insert

   TEV_SH
   

   Use

   Tags

   V5 His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mito cpVenus control

   Plasmid

   #186792

   Purpose

   Identical to mito NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   mito cpVenus control
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDest-527

   Plasmid

   #11518

   Purpose

   Gateway destination vector for bacterial expression; His tag

   Depositor

   Dominic Esposito

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCh-KIF5B*-strep

   Plasmid

   #120164

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member 5B (Kif5b Mouse)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIF5B: 1-555 aa

   Promoter

   CMV

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJR104

   Plasmid

   #187243

   Purpose

   Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-mCherry

    Plasmid

    #114472

    Purpose

    mCherry Control

    Depositor

    Karl Deisseroth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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