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psiCheck2 3xUTR

Plasmid

#40762

Purpose

Renilla luciferase reporter containing siRNA target sites

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

3 copies of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Promoter

TK/SV40

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
psicheck2 6xUTR

Plasmid

#40763

Purpose

Renilla luciferase reporter containing siRNA target sites

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

6 copies of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Promoter

TK/SV40

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pGFH14A6-12

Plasmid

#39543

Depositor

Katharina Strub

Article

Lakkaraju et al Cell. 2008 May 2;133(3):440-51.

Insert

SRP14 (SRP14 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 101-113

Promoter

CMV

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only
PM-ST1!TB

Plasmid

#48654

Purpose

Bacterial ST1 crRNA expression: targets ST1 to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial ST1 crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pBC2101-reci

Plasmid

#117170

Purpose

U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLS

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_wtCas9 with SV40 NLS

Use

Lentiviral

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pClodANL-FGF2YE

Plasmid

#107872

Purpose

Bacterial expression of FGF2phosphomimic-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

FGF2-phosphomimic

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Mutation

Y82E

Promoter

pro D

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMHEZFCNGA5

Plasmid

#69861

Purpose

oocyte expression vector containing ZFCNGA5

Depositor

Gary Matthews

Article

Tetreault et al Biochem Biophys Res Commun. 2006 Sep 22;

Insert

ZFCNGA5 (cnga5 Zebrafish)

Use

Xenopus oocyte expression

Mutation

none

Promoter

T7

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pMIG AS B56 gamma3

Plasmid

#13800

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

Anti-sense PP2A B56 gamma3 (PPP2R5C Human)

Use

Retroviral

Expression

Mammalian

Mutation

Anti-sense

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pLKO.1P shISCU

Plasmid

#102972

Purpose

Suppression of ISCU

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shISCU

Use

Lentiviral and RNAi

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 WT

Plasmid

#87599

Purpose

Encodes Prp5 WT for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 )

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-p110α(K227E)

Plasmid

#39491

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110(K227E) (PIK3CA Bovine)

Tags

myc

Expression

Mammalian

Mutation

K227E

Promoter

CMV

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
SK-YFP-ST1-B

Plasmid

#48662

Purpose

Bacterial ST1 repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

ST1 prototspacer B/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-ST1!TA

Plasmid

#48653

Purpose

Bacterial ST1 crRNA expression: targets ST1 to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial ST1 crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pSP64T BMPR (DM#136)

Plasmid

#15068

Depositor

Douglas Melton

Article

Graff et al Cell. 1994 Oct 7. 79(1):169-79.

Insert

BMP receptor (bmpr1a Frog)

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pAJ13

Plasmid

#14915

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

multiple cloning site 3

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pMV2 BMPR (DM#138)

Plasmid

#15069

Depositor

Douglas Melton

Article

Graff et al Cell. 1994 Oct 7. 79(1):169-79.

Insert

BMP receptor (bmpr1a Frog)

Expression

Mammalian

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pUASTattBVhhGFP4-Cry2

Plasmid

#244065

Purpose

antiGFP-nanobody fused to the light sensitive dimerization domain CRY2

Depositor

Markus Affolter

Article

Bao et al G3 (Bethesda). 2025 Jun 4;15(6):jkaf073.

Insert

VhhGFP4-Cry2-myc

Tags

myc

Expression

Insect

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pUASTattBRhokinase-CIBN

Plasmid

#244066

Purpose

active kinase domain of Rok fused to the light sensitive dimerization domain CIBN

Depositor

Markus Affolter

Article

Bao et al G3 (Bethesda). 2025 Jun 4;15(6):jkaf073.

Insert

Rhokinase-CIBN-HA

Tags

HA

Expression

Insect

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pMS05

Plasmid

#230069

Purpose

Inducible Promoter (anhydrotetracycline-induced, tetO/tetR system), replicates via RepA; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Bacterial

Promoter

anhydrotetracycline-inducible

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMS04

Plasmid

#230068

Purpose

Inducible Promoter (anhydrotetracycline-induced, tetO/tetR system), integrates via IntN1; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Bacterial

Promoter

anhydrotetracycline-inducible

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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