We narrowed to 19,027 results for: Tec

1. LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ

   Plasmid

   #192402

   Purpose

   On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   FRT-Act2-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ

   Plasmid

   #192405

   Purpose

   Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   B3RT-Act2-B3RT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ

   Plasmid

   #192403

   Purpose

   Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   FRT-Act2-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ

   Plasmid

   #192381

   Purpose

   To test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ

   Plasmid

   #192379

   Purpose

   To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpO

   Plasmid

   #192377

   Purpose

   To test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpO

   Plasmid

   #192378

   Purpose

   To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EK0544 CMV-TO-mCherry-SG(s70587-dblstop)SG-EGFP (FLP-IN)

   Plasmid

   #191176

   Purpose

   Mammalian expression of EK0209 with a second stop codon.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   CMV-TO:mCherry:s70587:EGFP (double-stop)
   

   Expression

   Mammalian

   Mutation

   additional stop codon

   Promoter

   CMV-TO

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EK0546 SFFV-mCherry-SGc(GAPDH.s1-dblstop)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191178

   Purpose

   Mammalian expression of EK0493 with second stop codon.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:GAPDH.s1:EGFP (double-stop)
   

   Expression

   Mammalian

   Mutation

   additional stop codon

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EK0344 SFFV-mCherry-SG(Bdnf.s1)SG-Cre (FLP-IN)

    Plasmid

    #191158

    Purpose

    Expression of a sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, Cre output; 90 bp long

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s1:Cre
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NSK025 CMV-TO-EGFP:c.637A>G(N213D; CCA>CCG)-stop-t70587 (PB)

    Plasmid

    #191166

    Purpose

    Mammalian expression of a EGFP mutant N213D derived from EK0379.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    EGFP(N213D)
    

    Expression

    Mammalian

    Mutation

    c.637A>G(N213D; CCA>CCG)

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EK0312 pAAV-EF1a-DIO-EYFP-WPRE-HGHpA

    Plasmid

    #191148

    Purpose

    AAV expression of a Cre reporter with EYFP output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    DIO-EYFP
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    hEF1a

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EK0362 SFFV-mCherry-SG(Bdnf.s1L36+54@202)SG-EGFP (FLP-IN)

    Plasmid

    #191153

    Purpose

    Mammalian expression of a 90 bp (36+54) long sensor for murine Bdnf 3' UTR where the trigger sequence is split

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s1L36+54:EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJCble

    Plasmid

    #185861

    Purpose

    The CRISPR/Cas9-and-gRNA-expressing plasmid with a phleomycin-resistent selectable marker

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; ble
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIALD2S

    Plasmid

    #185865

    Purpose

    Knocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    ALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pv6_dPC_2xChromo

    Plasmid

    #179398

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_2xChromo

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    dPC_2xChromo
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. rtTA-sg-mGreenLantern332

    Plasmid

    #188483

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern sgRNA
    

    Use

    CRISPR

    Tags

    PB_rtTA_BsmBI

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. rtTA-sg-CMV152

    Plasmid

    #188485

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. rtTA-sg-tdTomato166/892

    Plasmid

    #188488

    Purpose

    gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. rtTA-sg-tdTomato90/816

    Plasmid

    #188487

    Purpose

    gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge