We narrowed to 19,051 results for: Tec;

1. LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ

   Plasmid

   #192375

   Purpose

   To test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-35Ster-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LLP729_L2_pV01_0I_FRT-OCS-rLUC_TCTP-fLUC

   Plasmid

   #192372

   Purpose

   To test for the repressive potential of the OCS with the FRT sites around it. Then used as a control to represent the off state of many cicuits.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

   Plasmid

   #192374

   Purpose

   To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCStrunc-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ

   Plasmid

   #192385

   Purpose

   To test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::B3RT-OCS-B3RT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

   Plasmid

   #192386

   Purpose

   To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ

   Plasmid

   #192404

   Purpose

   On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   B3RT-Act2-B3RT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ

   Plasmid

   #192401

   Purpose

   Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::B3RT-Rluc-B3RT
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ

   Plasmid

   #192400

   Purpose

   On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::B3RT-Rluc-B3RT
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ

   Plasmid

   #192402

   Purpose

   On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   FRT-Act2-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ

    Plasmid

    #192405

    Purpose

    Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    B3RT-Act2-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ

    Plasmid

    #192403

    Purpose

    Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    FRT-Act2-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ

    Plasmid

    #192381

    Purpose

    To test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ

    Plasmid

    #192379

    Purpose

    To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpO

    Plasmid

    #192377

    Purpose

    To test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpO

    Plasmid

    #192378

    Purpose

    To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EK0544 CMV-TO-mCherry-SG(s70587-dblstop)SG-EGFP (FLP-IN)

    Plasmid

    #191176

    Purpose

    Mammalian expression of EK0209 with a second stop codon.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    CMV-TO:mCherry:s70587:EGFP (double-stop)
    

    Expression

    Mammalian

    Mutation

    additional stop codon

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EK0546 SFFV-mCherry-SGc(GAPDH.s1-dblstop)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191178

    Purpose

    Mammalian expression of EK0493 with second stop codon.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:GAPDH.s1:EGFP (double-stop)
    

    Expression

    Mammalian

    Mutation

    additional stop codon

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EK0344 SFFV-mCherry-SG(Bdnf.s1)SG-Cre (FLP-IN)

    Plasmid

    #191158

    Purpose

    Expression of a sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, Cre output; 90 bp long

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s1:Cre
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NSK025 CMV-TO-EGFP:c.637A>G(N213D; CCA>CCG)-stop-t70587 (PB)

    Plasmid

    #191166

    Purpose

    Mammalian expression of a EGFP mutant N213D derived from EK0379.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    EGFP(N213D)
    

    Expression

    Mammalian

    Mutation

    c.637A>G(N213D; CCA>CCG)

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EK0312 pAAV-EF1a-DIO-EYFP-WPRE-HGHpA

    Plasmid

    #191148

    Purpose

    AAV expression of a Cre reporter with EYFP output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    DIO-EYFP
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    hEF1a

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge