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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 6681 - 6700 of 14582 results

  2. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

    Plasmid
    #122850
    Purpose
    Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli
    Depositor
    Martin Grininger
    Publication
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    m(KS_MAT)_mouseL_ACP (Fasn Mouse)
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122850-plasmid-map-sequence-id-238544

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  3. pCAG-AaCas12b(D977A)-2AeGFP

    Plasmid
    #121962
    Purpose
    Mammalian expression, Transcription regulation, deactivated AaCas12b
    Depositor
    Wei Li
    Publication
    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Catalytically deactivated AaCas12b
    Tags
    3xHA tag
    Expression
    Mammalian
    Mutation
    D977A
    Available since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    121962-plasmid-map-sequence-id-235358

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  4. pTEI039

    Plasmid
    #105428
    Purpose
    Synthetic biology
    Depositor
    Johannes Stuttmann
    Publication
    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LexA-VP16-ER (transcription factor: inducible expression; estradiol)
    Use
    Synthetic Biology
    Mutation
    BsaI/ BpiI restriction sites removed
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105428-plasmid-map-sequence-id-449839

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  6. pUC19-F3UXG6

    Plasmid
    #103108
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    F3UXG6(Cas9 coding gene from Streptococcus sanguinis SK49)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103108-plasmid-map-sequence-id-201817

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  11. AAV2_hSyn_iChloC_CA_Citrine

    Plasmid
    #98220
    Purpose
    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Publication
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct iChloC_C128A gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    E83Q, E90R, E101S, C128A, T159C, D156N
    Promoter
    human synapsin
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    98220-plasmid-map-sequence-id-256053

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  14. pEYFP-N1-hRIP3-FL V460P

    Plasmid
    #61379
    Purpose
    Expresses Human full length RIPK3 with V460P mutation in the mammalian cells
    Depositor
    Francis Chan
    Publication
    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Full length human RIP3 with V460Pmutation (RIPK3 Human)
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    changed V460 to P
    Available since
    Jan. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pEYFP-N1-hRIP3-FL V458P

    Plasmid
    #61377
    Purpose
    Expresses Human full length RIPK3 with V458P mutation in the mammalian cells
    Depositor
    Francis Chan
    Publication
    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Full length human RIP3 with V458Pmutation (RIPK3 Human)
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    changed V458 to P
    Available since
    Jan. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 6681 - 6700 of 14582 results