Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 7,924 results for: mme

Clear keywords
Showing: 6701 - 6720 of 7924 results

  1. Antares-SEPLuc

    Plasmid
    #141086
    Purpose
    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    Antares-T2A-puromycin-EMCV IRES-SEPLuc
    Expression
    Mammalian
    Mutation
    Deletion of amino acids 66 to 91 on the Luciferas…
    Promoter
    CMV
    Available Since
    June 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    141086-plasmid-map-sequence-id-296862

    Enlarge

  2. pENTR-R4R3-stop

    Plasmid
    #113745
    Purpose
    Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    stop cassette
    Use
    CRISPR; Gateway entry vector
    Mutation
    Added 3 stop codons to the original sequence; see…
    Available Since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113745-plasmid-map-sequence-id-260896

    Enlarge

  3. mCherry-PhoCl AND A-SpyTag

    Plasmid
    #246950
    Purpose
    Expresses multifunctional construct for AND-gated release of mCherry from materials in response to 405 nm light AND sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag, SsrA
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246950-plasmid-map-sequence-id-489551

    Enlarge

  4. ACE2-Mutant-C4

    Plasmid
    #240608
    Purpose
    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 4 located within the collectrin-like site on ACE2
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Arginine (R) and Lysine (K) residues on ACE2-clus…
    Promoter
    CMV
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    240608-plasmid-map-sequence-id-483854

    Enlarge

  8. pUC-T7-hTP53-P0~670-FLuc

    Plasmid
    #208640
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.
    Depositor
    Quanfu Ma
    Insert
    TP53, UTR and partial CDS (TP53 Human)
    Use
    Luciferase; In vitor transcription template
    Tags
    Firefly Luciferase (F-Luc) and T7 promoter
    Mutation
    UTR and partial CDS in-frame with F-Luc
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208640-plasmid-map-sequence-id-418347

    Enlarge

  9. IBMc397

    Plasmid
    #161955
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161955-plasmid-map-sequence-id-326361

    Enlarge

  10. pB6-Id1-loxP-AU1-rox-FNF-loxP-drePBD*-rox

    Plasmid
    #168770
    Purpose
    B6J Id1 AU1 tag and LSL-TagRFP-T-drePBD* targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Id1 (Id1 Mouse)
    Use
    Cre/Lox and Mouse Targeting; Dre / rox
    Mutation
    See Depositor Comments Below
    Available Since
    Aug. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    168770-plasmid-map-sequence-id-337553

    Enlarge

  14. pSWD95D293A

    Plasmid
    #159099
    Purpose
    CckA FRET Sensor with DITE motif mutant
    Depositor
    Seth Childers
    Article
    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do
    Insert
    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
    Expression
    Bacterial
    Mutation
    CckA D293 mutated to A293 and V199I on mRuby3 (P…
    Promoter
    Xylose
    Available Since
    Nov. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159099-plasmid-map-sequence-id-316589

    Enlarge

  16. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid
    #159508
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(0)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159508-plasmid-map-sequence-id-311756

    Enlarge

  17. pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid
    #159499
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-2_PolyU(1)_HairpinDistance(0)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159499-plasmid-map-sequence-id-311745

    Enlarge

  18. pAV-U6+27-Linear-1_PolyU(4) _HairpinDistance(0)_Tornado-Corn

    Plasmid
    #159496
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-1_PolyU(4) _HairpinDistance(0)
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159496-plasmid-map-sequence-id-311762

    Enlarge

  19. pAV-U6+27-Linear-1_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid
    #159495
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-1_PolyU(1)_HairpinDistance(0)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159495-plasmid-map-sequence-id-311767

    Enlarge

Showing: 6701 - 6720 of 7924 results