We narrowed to 8,222 results for: mme
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Plasmid#225154PurposeMedium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only
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pAF146
Plasmid#222377PurposeExpress Tasmanian devil PDL1 protein (saha_PDL1_FL_DN11026_c0_g1_i1)DepositorInsertPDL1
ExpressionMammalianPromoterEF1aAvailable SinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP DAmP
Plasmid#182511PurposeC-SWAT compatible vector for C-terminal tagging with GFP DAmP. GFP is immediately followed by the Hygromycin B (Hph) resistance cassette.DepositorInsertGFP
TagsGFPExpressionYeastAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-brwd1
Plasmid#192742PurposeGateway entry vector encoding zebrafish brwd1DepositorInsertbrwd1 (brwd1 Zebrafish)
UseGateway CloningMutationK144W; D145E; I760T (rs512641576); Y770F (rs50744…PromoterNoneAvailable SinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIT6EG7mlh
Plasmid#185887PurposeIn vivo gene amplification (HapAmp) of limonene synthetic module at RPL25 locus (23 copy).DepositorInsertsee comments
ExpressionYeastMutationERG20 F96W N127WAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILAC2
Plasmid#185889PurposeIn vivo gene amplification (HapAmp) of lycopene synthetic module at RPL25 locusDepositorInsertsee comments
ExpressionYeastMutationERG20 F96C; Xd.CRtYB E83KAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzBST
Plasmid#172968PurposepWSK29 with Salmonella typhimurium wzzBST geneDepositorInsertwzzB gene from Salmonella enterica serovar Typhimurium
ExpressionBacterialMutationK198E- please see depositor commentsAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-fepEST
Plasmid#172969PurposepWSK29 with Salmonella typhimurium fepEST geneDepositorInsertfepE gene from Salmonella enterica serovar Typhimurium
ExpressionBacterialMutationG160S- please see depositor commentsAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E26: attB4-SV40p>-attB1r
Plasmid#162923PurposeGateway attB4/attB1r entry clone for mammalian expression using the SV40 promoterDepositorInsertSV40 immediate early promoter
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-D1135E
Plasmid#154924PurposeaTc inducible Cas9HF1 with D1135E mutationDepositorInsertCas9HF1-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationN497A/R661A/Q695A/Q926A/D1135E and stop codon on …PromoterpTetAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEE22
Plasmid#161787PurposeHi-SC2 reporter delivery plasmidDepositorInsertHi-SC2 reporter
UseConjugative plasmid to deliver hi-sc2 reporterMutationHimar site included MmeI site, tandem loxP sites,…Promotern/aAvailable SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pISH-Olfr1395
Plasmid#136731PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH) for Olfr1395DepositorInsertOlfr1395
UseIn situ probeMutationISH Probe insert, D175G and L220F- please dep. co…Available SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-lambdaN-UPF1-DEAA
Plasmid#136007PurposeDEAA UPF1 inserted with lambdaN tagged on the N-terminus to use with the Tethering Assay (BoxB)DepositorInsertUPF1
TagsLambdaNExpressionMammalianMutationDE647AA and S743A (Please see depositor comments …Available SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMLS415
Plasmid#114272PurposeSapTrap donor vector for point mutagenesis; pENTR-221::syntron(lox2272-119+)DepositorInsertCbr-Unc-119
UseCRISPR and Cre/LoxMutation*please see depositor comment belowAvailable SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGolt1-mCherry
Plasmid#73361PurposeIn vivo protein protein interaction studies/ expression in mammalian cellsDepositorInsertTMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
TagsmCherryExpressionMammalianPromoterCMV immediate early promoterAvailable SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGolt2-mCherry
Plasmid#73358PurposeIn vivo protein protein interaction studies/ expression in mammalian cellsDepositorInsertTMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
TagsmCherryExpressionMammalianPromoterCMV immediate early promoterAvailable SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-Fv2E-flt4-ns
Plasmid#67695Purposemiddle entry plasmid with dimerizable extracellular domain fused to Danio Flt4 cytoplasmic domainDepositorInsertFv2E-flt4
Mutationno stop codon; D1302E (see depositor's comme…Available SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpDJ-1 pET15b
Plasmid#60689PurposeRecombinant protein expression of DJ-1DepositorAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
4MI0
Plasmid#60097PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertEZH2:JMC033-A03:C216172 (EZH2 Human)
UseBaculovirus expressionTags6xHisMutationSee commentsPromoterT7Available SinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
FLAG-Sec31a W985A #790
Plasmid#42122DepositorInsertSec31a (SEC31A Human)
TagsFLAGExpressionMammalianMutationG742R and W985A NOTE: the mutation is actually W…PromoterCMVAvailable SinceMarch 12, 2013AvailabilityAcademic Institutions and Nonprofits only