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pcDNA-Nup37-EGFP-polyA

Plasmid

#104562

Purpose

Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Nup37 (Nup37 Mouse)

Tags

EGFP tag

Expression

Mammalian

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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MP6-SlugABEN-738

Plasmid

#214348

Purpose

Expresses N-terminus of ABE, and untargeted Bacterial Mutagenesis

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

dnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE

Expression

Bacterial

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-scFv-p65-HSF1-Blast

Plasmid

#192652

Purpose

3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

scFv-p65-HSF1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQAT59

Plasmid

#223431

Purpose

T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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HA-AMOT p130 Y242/287A

Plasmid

#32822

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

HA

Expression

Mammalian

Mutation

E5D, Y242/287A

Promoter

CMV

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
pBEL1092

Plasmid

#195635

Purpose

Expression/purification of 6xHis-TEV-sfGFP

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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Myc-CICf

Plasmid

#48185

Purpose

Expresses Cic

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Capicua (Cic Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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EB3-3xmScarletI-N1

Plasmid

#112928

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

EB3

Tags

3x mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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SERA9-bio-His

Plasmid

#50820

Purpose

Expresses enzymatically monobiotinylated full length SERA9

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA9

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 12, 2014

Availability

Academic Institutions and Nonprofits only

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pETCON-PTX-S1-220

Plasmid

#67747

Purpose

Pertussis Toxin Subunit 1 Yeast Display Plasmid

Depositor

Timothy Whitehead

Article

Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

Insert

PTxS1-220

Expression

Bacterial and Yeast

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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[UBC][NRE Pum][eGFP]

Plasmid

#97192

Purpose

The wild-type Pum, fused to eGFP, and behind the ubiquitin promoter.

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Insert

wild type Pumilio homology domain

Use

Synthetic Biology

Tags

eGFP

Expression

Mammalian

Promoter

UBC (ubiquitin C)

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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PKG I beta WT

Plasmid

#16394

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 beta (PRKG1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Zeb2-3'UTR

Plasmid

#61771

Purpose

Luciferase reporter containing the 3'UTR of mouse Zeb2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Zeb2 3'UTR

Use

Luciferase

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-SlugCas9-P2A-puro

Plasmid

#214353

Purpose

Expresses SlugCas9 and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCas9 and puromycin resistance genes

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5 Myc-RIP1

Plasmid

#44159

Depositor

Xin Lin

Article

Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Insert

RIP1 (RIPK1 Human)

Tags

Myc

Expression

Mammalian

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-Sa-SauriCas9

Plasmid

#135967

Purpose

Expresses Sa-SauriCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pP35S (GB0030)

Plasmid

#68163

Purpose

Provides the CaMV 35S Promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

CaMV 35S promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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MSRP4-bio-His

Plasmid

#50806

Purpose

Expresses enzymatically monobiotinylated full length MSRP4

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

MSRP4

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PFA0210c-bio-His

Plasmid

#50821

Purpose

Expresses enzymatically monobiotinylated full length PFA0210c

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PFA0210c

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pET-His-Dre

Plasmid

#123130

Purpose

Bacterial expression plasmid for wild-type Dre with an N-terminal His-tag.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Dre recombinase

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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