We narrowed to 9,393 results for: sacs

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pLyGo-Ec-6

Plasmid

#163132

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pTH353-SUP45wt

Plasmid

#29371

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

Wild type gene including genomic sequence 1432 bp…

Available Since

May 5, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX4T2-Snf1-cat

Plasmid

#52683

Purpose

expression of the Snf1 kinase domain in bacteria

Depositor

Benjamin Turk

Article

Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

Insert

SNF1 (SNF1 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

encodes kinase domain only, aa 41-315

Promoter

tac

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pDUAL-Pnmt1-Ost4-GFP-MTn

Plasmid

#154369

Purpose

Yeast expression of Ost4

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Ost4 (ost4 Fission Yeast)

Expression

Yeast

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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RP78 pUAST Orco mRNA

Plasmid

#53431

Purpose

Expresses the full-length Drosophila melanogaster Orco cDNA under Gal4/UAS control

Depositor

Leslie Vosshall

Article

Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

Insert

Orco (Orco )

Expression

Insect

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only
pRS406 ATG1-ATG17-3'UTR

Plasmid

#166848

Purpose

Expresses Atg1 with ATG17 3'UTR in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Promoter

ATG1 Promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Kp-2

Plasmid

#163144

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Promoter

AOX1

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-16-Decoy.hPGK.GFP.WPRE

Plasmid

#46593

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-16 Decoy (Mir16-1 Human, Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
POT11-RFP

Plasmid

#65327

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pRS424-URR

Plasmid

#65330

Purpose

high copy number plasmid as receiving vector for exogenous pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

URR1-RFP-Leu2-URR2

Use

Synthetic Biology

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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POT5-RFP

Plasmid

#65321

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pRS414-URR

Plasmid

#65329

Purpose

CEN plasmid as receiving vector for exogenous pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

URR1-RFP-Leu2-URR2

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMV-URR2

Plasmid

#65332

Purpose

standard plasmids for exogenus pathway assembly haboring recombination sequence 2 (Universal Recombination Region 2) which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

URR2

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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POT3-RFP

Plasmid

#65319

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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POT9-RFP

Plasmid

#65325

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMV-URR1

Plasmid

#65331

Purpose

standard plasmids for exogenus pathway assembly haboring recombination sequence 1(Universal Recombination Region 1) which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

URR1

Use

Synthetic Biology

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pAc-HT-Dmp53

Plasmid

#85698

Purpose

expression of Drosophila p53 in Drosophila cells

Depositor

Licio Collavin, Giannino Del Sal

Article

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

Dmp53 (p53 Fly)

Tags

His

Expression

Insect

Promoter

Ac5

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pILGFP86

Plasmid

#83543

Purpose

Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL4A promoter-yEGFP

Expression

Yeast

Promoter

RPL4A

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2800

Plasmid

#63657

Purpose

pCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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ptagBFP-TetR-NLS-VP16

Plasmid

#103835

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

tagBFP-TetR-NLS-VP16 (tetR )

Expression

Mammalian

Promoter

SV2

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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