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pmTurquoise-C1

Plasmid

#60558

Purpose

Expresses the cyan fluorescent protein variant mTurquoise

Depositor

Dorus Gadella

Article

Goedhart et al Nat Methods. 2010 Feb;7(2):137-9. doi: 1

Insert

mTurquoise

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pDH013

Plasmid

#157638

Purpose

sMHT-113 fused to GFP and RFP

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17-GFP
RFP-SYNZIP18-sMHT(114-23)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-clbH-A1

Plasmid

#114149

Purpose

Expresses ClbH-A1

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbH-A1

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Contains only the A1 domain of ClbH

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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ins-cre_cryaa-Venus

Plasmid

#24333

Depositor

Didier Stainier

Article

Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

Insert

Cre (cre )

Use

Cre/Lox

Tags

Venus

Expression

Worm

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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EcYbbN pET15b

Plasmid

#60690

Purpose

Recombinant protein expression of YbbN

Depositor

Mark Wilson

Article

Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

Insert

YbbN

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA TfR1-miniE

Plasmid

#181987

Purpose

MiniE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

TfR1-MiniE

Expression

Mammalian

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pL2-(GGS)2G

Plasmid

#137097

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

(GGS)2G

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-GGASPAAPAPAG

Plasmid

#137103

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GGASPAAPAPAG

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-G(TP)4TG

Plasmid

#137101

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

G(TP)4TG

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-(GGS)3G

Plasmid

#137098

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

(GGS)4G

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-GGSG

Plasmid

#137095

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GGSG

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-GPPPG

Plasmid

#137094

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GPPPG

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-GPG

Plasmid

#137093

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GPG

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-GSPAG

Plasmid

#137096

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GSPAG

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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DS-ST1cas

Plasmid

#48647

Purpose

Bacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
ST1 tracrRNA

Use

CRISPR

Promoter

proC

Available Since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt16

Plasmid

#42291

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt16 (Wnt16 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pcDXC3GMS

Plasmid

#49031

Purpose

FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptide

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), EGFP, MYC, a…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMTP170 (pBAD33_CRISPR(Tn6900)-entry)

Plasmid

#164263

Purpose

Entry vector for cloning user-defined spacers in the Tn6900 CRISPR array.

Depositor

Joseph Peters

Article

Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

Type

Empty backbone

Expression

Bacterial

Promoter

Arabinose

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pJSK619

Plasmid

#205134

Purpose

Expression of SnxA-GFP in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSuperRetro-EFP siRNA2

Plasmid

#12450

Depositor

Dong-Er Zhang

Article

Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94

Insert

siRNA against EFP (TRIM25 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only