We narrowed to 13,379 results for: mpl

1. pcDNA3.3−3xFLAG−AGO2(D669A)

   Plasmid

   #220461

   Purpose

   Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Human AGO2 (AGO2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Aspartate 669 to alanine

   Promoter

   CMV

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. plenti-UBC-HA-TMEM230

   Plasmid

   #225516

   Purpose

   Lentiviral TMEM230 expression vector

   Depositor

   Wade Harper

   Article

   Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

   Insert

   TMEM230 (TMEM230 Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-CHC17KDP

   Plasmid

   #59799

   Purpose

   Expresses knockdown-proof human CHC17 tagged with GFP.

   Depositor

   Stephen Royle

   Article

   Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

   Insert

   CHC17 (CLTC Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Silent mutations in amino acids 61-65 to confer s…

   Promoter

   CMV

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-itis pBE-t

   Plasmid

   #195342

   Purpose

   Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V

   Depositor

   Alexis Komor

   Article

   Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

   Inserts

   ecTadA(8e)-SpCas9-NG
   gRNA ctctacgcgggtcttgtagt
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

   Promoter

   Lac and ProC

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

   Plasmid

   #127353

   Purpose

   gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and mVenus

   Expression

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-miRFP670nano3-Blast-H2BC11

   Plasmid

   #207762

   Purpose

   Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCDFDuet-nsp7-nsp8

   Plasmid

   #159092

   Purpose

   Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

   Depositor

   Seth Darst

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Inserts

   NSP7
   NSP8
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-itis pBE-nt

   Plasmid

   #195343

   Purpose

   Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA

   Depositor

   Alexis Komor

   Article

   Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

   Inserts

   ecTadA(8e)-SpCas9-NG
   gRNA gtgcacgacgccgtatgcga
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

   Promoter

   Lac and ProC

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-SynI-CreOn-FlpOff-TeNT-P2A-EGFP

   Plasmid

   #176282

   Purpose

   Viral vector for co-expression of TeNT and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   TeNT-P2A-EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin I

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. NSP4-WT-Flag

    Plasmid

    #210339

    Purpose

    Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-LMO7 (mNeonGreen-eKL9h-VChR1)

    Plasmid

    #205097

    Purpose

    fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Bjorefeldt et al bioRxiv. 2023 Dec 4:2023.06.26.545546. d

    Insert

    mNeonGreen, eKaz luciferase, Volvox Channelrhodopsin 1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. R4pGWB601_UBQ10p-miniTurbo-NES-YFP

    Plasmid

    #127369

    Purpose

    Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA4.TO-ORF50-2xCSTREP

    Plasmid

    #136211

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF50

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF50
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. SNAP-BAF

    Plasmid

    #227443

    Purpose

    Mammalian expression of SNAP-BAF

    Depositor

    David Pellman

    Article

    Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

    Insert

    Barrier-to-autointegration factor (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMH-SFB-BRCA1

    Plasmid

    #99394

    Purpose

    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA1

    Depositor

    Michael Huen, Junjie Chen

    Article

    Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 barr1 flag

    Plasmid

    #14687

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    beta arrestin 1 (Arrb1 Rat)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pENTR221-TCF7

    Plasmid

    #79498

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    TCF7 (TCF7 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cav2.2 pcDNA3

    Plasmid

    #206072

    Purpose

    expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

    Depositor

    Annette Dolphin

    Article

    Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

    Plasmid

    #127355

    Purpose

    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and mVenus

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. qTAG-C-moxGFP-Zeo-H2BC11

    Plasmid

    #207759

    Purpose

    Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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