We narrowed to 8,708 results for: sgRNA

1. pYPQAT33

   Plasmid

   #223405

   Purpose

   T-DNA vector for SpCas9-D10A based A-to-G base editing for dicot plants; NGG PAM; SpCas9D10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-ecTadA8e-SpCas9-D10A-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYPQAT34

   Plasmid

   #223406

   Purpose

   T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpCas9-D10A-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQAT35

   Plasmid

   #223407

   Purpose

   T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpCas9-D10A-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYPQAT36

   Plasmid

   #223408

   Purpose

   T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpCas9-D10A-ZmUbi-gRNA scaffold-tRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEIF3D-WT-sgNT

   Plasmid

   #222120

   Purpose

   EIF3D overexpression vector with non-targeting sgRNA

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   eIF3D (EIF3D Human)
   

   Use

   Lentiviral

   Tags

   BFP-P2A

   Expression

   Mutation

   Promoter

   Available since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGM65

   Plasmid

   #220170

   Purpose

   CRISRPi guide RNA against NACA

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   NACA (sgRNA) (NACA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLentiCRISPRRv2_sgTom

   Plasmid

   #218346

   Purpose

   CRISPR KO plasmid for tdTomato in human cell lines

   Depositor

   Seth Parker

   Article

   Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

   Insert

   Inserted sgRNA targetting tdTomato
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGM133

   Plasmid

   #220173

   Purpose

   CRISRPi guide RNA against TTC1

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   TTC1 (sgRNA) (TTC1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV2ss-U6-sgGFP

   Plasmid

   #190899

   Purpose

   AAV vector expressing sgGFP

   Depositor

   Nicole Déglon

   Article

   Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

   Insert

   sgRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

    Plasmid

    #192131

    Purpose

    Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp1-Hsp2Cas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDe_Cas9_KANA_R160a

    Plasmid

    #173756

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160a

    Depositor

    Kristina Gruden

    Article

    Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

    Insert

    Cas9_sgRNA1/R160a_sgRNA2/R160a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6 &StU6

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDe_Cas9_KANA_R160b

    Plasmid

    #173757

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160b

    Depositor

    Kristina Gruden

    Article

    Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

    Insert

    Cas9_sgRNA1/R160b_sgRNA2/160b
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6 &StU6

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDe_Cas9_KANA_R390a

    Plasmid

    #173758

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA390a

    Depositor

    Kristina Gruden

    Article

    Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

    Insert

    Cas9_sgRNA1/390a_sgRNA2/390a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6 &StU6

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. dora[B]_rescue_construct

    Plasmid

    #190637

    Purpose

    Puromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Changed threonine 295 to methionine

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. dora[A]_rescue_construct

    Plasmid

    #190639

    Purpose

    Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Truncated to contain amino acids 1-945

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEPQD0CM0033

    Plasmid

    #185624

    Purpose

    Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-CTCG

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    NbU6-2
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEPQD0CM0032

    Plasmid

    #185623

    Purpose

    Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-TTCG

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    NbU6-1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK434

    Plasmid

    #155378

    Purpose

    PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK422

    Plasmid

    #155373

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK423

    Plasmid

    #155374

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge