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We narrowed to 9,204 results for: sgRNA

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Showing: 6741 - 6760 of 9204 results

  3. BB3cK_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104908
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    104908-plasmid-map-sequence-id-206067

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  17. pNSU299_YDR344C

    Plasmid
    #166072
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA targeting intergenic site near HXT6 (within YDR344C) for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Intergenic site near HXT6 (in YDR344C, possible dubious ORF)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166072-plasmid-map-sequence-id-329603

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  18. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid
    #227274
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227274-plasmid-map-sequence-id-452035

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  20. PB-2G-CNCB_sgRBBP6-68

    Plasmid
    #237555
    Purpose
    A piggybac-based vector containing mouse U6 promoter-driven RBBP6 sgRNA #6, human U6 promoter-driven RBBP6 sgRNA #8 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.
    Depositor
    Kazutoshi Takahashi
    Publication
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Insert
    RBBP6 (RBBP6 Human)
    Use
    Piggybac
    Expression
    Mammalian
    Promoter
    mouse U6 promoter and mouse U6 promoter, human U6…
    Available since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    237555-plasmid-map-sequence-id-473543

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Showing: 6741 - 6760 of 9204 results