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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,392 results for: crispr grnas

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Showing: 6761 - 6780 of 8392 results

  1. pMEL15_MATz

    Plasmid
    #166099
    Purpose
    Expression of gRNA targeting the MAT locus
    Depositor
    Rebecca Burgess
    Insert
    MAT Z1
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166099-plasmid-map-sequence-id-328795

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  12. B-SpCas9-HF1

    Plasmid
    #126762
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; Q926A; amino acids 1005-1013…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126762-plasmid-map-sequence-id-410965

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  13. P35m

    Plasmid
    #120946
    Purpose
    MoClo golden gate assembly AB part for pLbda-CasRQi (strong lambda promoter with downstream dCas binding site for Qi-lab gRNA (UC16m); gRNA sequence taken from DOI: 10.1016/j.cell.2013.02.022).
    Depositor
    Richard Murray
    Insert
    pLbda-CasRQi
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120946-plasmid-map-sequence-id-235948

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  18. pCfB6631

    Plasmid
    #106161
    Purpose
    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Insert
    unknown
    Expression
    Yeast
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106161-plasmid-map-sequence-id-206246

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  19. pCfB6630

    Plasmid
    #106160
    Purpose
    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6371 into Yarrowia lipolytica chromosomal location IntC_3, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Insert
    unknown
    Expression
    Yeast
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106160-plasmid-map-sequence-id-206239

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Showing: 6761 - 6780 of 8392 results