We narrowed to 4,845 results for: AAT

1. PLKO.1-G3BP1-CDS

   Plasmid

   #136039

   Purpose

   G3BP1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGGGAATTTGTGAGACAGTAT)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGPA1

   Plasmid

   #115476

   Purpose

   Generation of mariner-based transposon mutant libraries in Enterococcus faecium

   Depositor

   Willem van Schaik

   Article

   Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-TRPV1exCellHalo

   Plasmid

   #220307

   Purpose

   Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

   Depositor

   Eric Senning

   Article

   Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231

   Insert

   rTRPV1exCellHalo (Trpv1 Rat)
   

   Use

   Tags

   circularly permutated Halotag (Deo et al., Nat Ch…

   Expression

   Mammalian

   Mutation

   linker included to insert cpHaloTag (

   Promoter

   CMV promoter

   Available since

   July 30, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pRluc-LC3wt

   Plasmid

   #105002

   Purpose

   Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

   Depositor

   Marja Jããttelã

   Article

   Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Luciferase

   Tags

   Rluc with C124A substitution

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGCP123-GFP_g1

   Plasmid

   #153518

   Purpose

   sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

   Depositor

   Kimberly Kline

   Article

   Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

   Insert

   PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. L1_lacZgRNA-Ck3

   Plasmid

   #136136

   Purpose

   L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpU6:lacZgRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PLKO.1-G3BP1-3'UTR

   Plasmid

   #136038

   Purpose

   G3BP1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCTGTAAGAAATACAGGATT)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. L1_lacZgRNA-Ck2

   Plasmid

   #136137

   Purpose

   L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpU6:lacZgRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFEndoNA

   Plasmid

   #174724

   Purpose

   Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

   Depositor

   Jukka Finne

   Article

   Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

   Insert

   GFP-endoNA fusion protein
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-sh-mSOX9-2

    Plasmid

    #40645

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSOX9-2 (Sox9 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pQCXIP TBRII

    Plasmid

    #19147

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    Transforming growth factor, beta receptor II (TGFBR2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pLKO mouse shRNA 2 raptor

    Plasmid

    #21340

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    raptor shRNA 2 (Rptor Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

13. GFP-PKI WT

    Plasmid

    #118300

    Purpose

    Expression of PKIa

    Depositor

    Philip Stork

    Article

    Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

    Insert

    PKI wt
    

    Use

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJ23119-sgRNA

    Plasmid

    #113654

    Purpose

    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    promoter J23119 and sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

    Plasmid

    #197952

    Purpose

    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pcoCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26 and pUBQ10

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PLKO.1-CAPRIN1-3'UTR

    Plasmid

    #136055

    Purpose

    CAPRIN1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCACGTTAGTGTCACAAATT)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    CAPRIN1 (CAPRIN1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid

    #160139

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRDA_834

    Plasmid

    #216087

    Purpose

    Cas9 [Sp] knockout targeting CD59, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD59 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9(BB)-2A-GFP-G3BP2(1)

    Plasmid

    #136060

    Purpose

    G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP2 (G3BP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. SFG.CNa12_opt.IRES.eGFP

    Plasmid

    #22490

    Depositor

    Martin Pule

    Article

    Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

    Insert

    Codon optimised Calcineurin A mutant 12 (PPP3CA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    T351E; L354A

    Promoter

    Available since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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