We narrowed to 752 results for: Numb

1. pEMS1927

   Plasmid

   #79776

   Purpose

   High copy number pUC vector containing Ple254 (PAX6 MiniPromoter) insert

   Depositor

   Elizabeth Simpson

   Article

   Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

   Insert

   Ple254
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Expression

   Mutation

   Promoter

   Ple254 (PAX6 MiniPromoter)

   Available since

   Sept. 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pSG5-FLAG-mEKLF Zinc Fingers

   Plasmid

   #67837

   Purpose

   Expression of murine EKLF Zinc Fingers (aa 287-end) fused to Flag and driven by SV40 promoter

   Depositor

   James Bieker

   Article

   Chen et al Mol Cell Biol. 2001 May;21(9):3118-25.

   Insert

   EKLF (Klf1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Zinc Fingers are amino acids 287-end; this number…

   Promoter

   SV40

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

3. 5mARF17 pBluescript

   Plasmid

   #12069

   Depositor

   David Bartel

   Article

   Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

   Insert

   5mARF17 (ARF17 Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   miR160-resistant version of ARF17 has five silent…

   Promoter

   Available since

   June 2, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3 Flag MFF iso2 S172A

   Plasmid

   #74392

   Purpose

   Flag tagged human S172A MFF isoform 2 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser 172 Ala (numbering based on MFF isoform 1)

   Promoter

   Available since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pET30a-His6-ABP-LOXCATmut

   Plasmid

   #139972

   Purpose

   A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

   Depositor

   Vamsi Mootha

   Article

   Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Insert

   A fusion of lactate oxidase from A. viridans and catalase from E. coli
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutation in H265A and R268A in LOX (used original…

   Promoter

   T7

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG416GAL-ccdB-mRuby3

   Plasmid

   #166139

   Purpose

   Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRB051_LEU2

   Plasmid

   #196993

   Purpose

   Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

   Depositor

   Matthew Maurano

   Article

   Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUSEss-CHIg-mG1_Arg322Lys

   Plasmid

   #105849

   Purpose

   Expression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg322Lys (EU numbering).

   Depositor

   Joanna Bereta

   Article

   Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

   Insert

   IgG1 heavy chain (Ighg1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Arg322Lys in constant region of IgG1 heavy chain

   Promoter

   hEF1-HTLV

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-mkgDUX4mal*leu*

   Plasmid

   #21173

   Depositor

   Stephen Tapscott

   Article

   Snider et al Hum Mol Genet. 2009 Apr 9. ():.

   Insert

   DUX4 (DUX4 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   First point mutation at nucleotide 5114 (numberin…

   Promoter

   Available since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pCS2-mkgDUX4mal*mqg*

    Plasmid

    #21174

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Apr 9. ():.

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    First point mutation at nucleotide 5114 (numberin…

    Promoter

    Available since

    Aug. 6, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pEMS1920

    Plasmid

    #82562

    Purpose

    High copy number plasmid containing Ple253 (PITX3 MiniPromoter) insert.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Ple253
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pcDNA3 Flag MFF iso4 S172A

    Plasmid

    #74393

    Purpose

    Flag tagged human S172A MFF isoform 4 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pEMS1941

    Plasmid

    #82564

    Purpose

    High copy number plasmid containing Ple264 (NR2E1 MiniPromoter) insert.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Ple264
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pAG426GAL-ccdB-mRuby3

    Plasmid

    #166142

    Purpose

    Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a 2um element for high copy number when in yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HIS_TEV_hsSYX3

    Plasmid

    #179290

    Purpose

    E. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Use

    Tags

    His6 + TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    residues 393-792 from accession number NP_0010361…

    Promoter

    T7lac

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330_gRNA_H19-DMR-2

    Plasmid

    #159930

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CBh

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330_gRNA_H19-DMR-1

    Plasmid

    #159929

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CBh

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pB6-Lrig1-T2A-sfGFP-iCre-ERT2-FNF-TK

    Plasmid

    #126651

    Purpose

    B6J Lrig1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    Lrig1 (Lrig1 Mouse)
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    C-terminal fusion of T2A-sfGFP-iCre-ERT2

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. 35S:5mARF17 pLBR19

    Plasmid

    #12073

    Depositor

    David Bartel

    Article

    Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

    Insert

    5mARF17 (ARF17 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    miR160-resistant version of ARF17 has five silent…

    Promoter

    Available since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pTT5-hSDC1

    Plasmid

    #52326

    Purpose

    expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R117Q mutation (R95Q according to numbering in Fi…

    Promoter

    CMV

    Available since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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