We narrowed to 1,476 results for: transfection

1. pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

   Plasmid

   #113041

   Purpose

   AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. IFITM3-N21del-V93-TAG

   Plasmid

   #122480

   Purpose

   expresses human IFITM3-N21del with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

   Depositor

   Howard Hang

   Article

   Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

   Insert

   IFITM3 (IFITM3 Human)
   

   Expression

   Mammalian

   Mutation

   Changed Valine 93 to amber codon and deleted amin…

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)

   Plasmid

   #113040

   Purpose

   AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   gRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

   Plasmid

   #113042

   Purpose

   AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-IFITM3-F8-TAG-C105A

   Plasmid

   #122478

   Purpose

   expresses human IFITM3-C105A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

   Depositor

   Howard Hang

   Article

   Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

   Insert

   IFITM3 (IFITM3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Phenylalanine 8 to amber codon, cysteine …

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-IFITM3-V93-TAG

   Plasmid

   #122479

   Purpose

   expresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

   Depositor

   Howard Hang

   Article

   Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

   Insert

   IFITM3 (IFITM3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Valine 93 to amber codon

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-IFITM3-F8-TAG-C71A

   Plasmid

   #122476

   Purpose

   expresses human IFITM3-C71A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

   Depositor

   Howard Hang

   Article

   Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

   Insert

   IFITM3 (IFITM3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Phenylalanine 8 to amber codon, cysteine …

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. BMP10 N67Q N131Q D4G (S2-2)

   Plasmid

   #184622

   Purpose

   for transfection into S2 cells and expression of BMP10 protein

   Depositor

   Timothy Springer

   Article

   Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

   Insert

   BMP10 (BMP10 Human)
   

   Tags

   8x His - streptavidin binding peptide

   Expression

   Insect

   Mutation

   N67Q, N131Q, ARIRR(312-316) replaced with G

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHcRed-NPM1cdN243-C1

   Plasmid

   #131838

   Purpose

   Expresses the last 50 amino acids of HcRed-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Tags

   HcRed

   Expression

   Mammalian

   Mutation

   This is a deletion contruct of the cytoplasmic m…

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHcRed-NPM1cOligodN243-C1

    Plasmid

    #131847

    Purpose

    Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the oligo dimerization domain attached to the last 50 aminoacids in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    This is a deletion contruct of the cytoplasmic m…

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHcRed-NPM1cNLSdN243-C1

    Plasmid

    #131848

    Purpose

    Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the NLS domain (aa. 121-195) attached to the last 50 aminoacids in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    This is a deletion contruct of the cytoplasmic m…

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHcRed-NPMMLF1nes-C1

    Plasmid

    #131849

    Purpose

    Expresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    This is a control deletion contruct of the cytop…

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PuroR-T2A-eGFP

    Plasmid

    #234734

    Purpose

    Puromycin resistance/eGFP cassette in mammalian cells (transfection marker)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    PuroR-T2A-eGFP
    

    Expression

    Mammalian

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCXWB-EBNA1

    Plasmid

    #37624

    Purpose

    Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    EBNA-1 (EBNA-1 human herpesvirus 4)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid

    #227273

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid

    #227274

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. WDR59 in pRK5

    Plasmid

    #154214

    Purpose

    Untagged WDR59 cDNA expression vector for transfections

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    WDR59 (WDR59 Human)
    

    Tags

    NONE

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRES_IBB-mCherry-IRES-HA-mAID-nanobody

    Plasmid

    #117720

    Purpose

    Expression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Inserts

    mAID-nanobody
    IBB-mCherry
    

    Tags

    importin-beta bining domain (IBB) and triple HA

    Expression

    Mammalian

    Mutation

    codon optimized mAID for murine expression

    Promoter

    CMV and None, IRES-driven

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAG-mCherry

    Plasmid

    #108685

    Purpose

    Strong CAG promoter with mCherry expression for transfection efficacy screening

    Depositor

    Jordan Green

    Article

    Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

    Insert

    mCherry
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid

    #221004

    Purpose

    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    CasRx 30nt processed direct repeat with SD1 mutation
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    DR1 A>T

    Promoter

    U6

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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