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pCAG-miRFP670-pA_U6-BbsI (pE059)

Plasmid

#149353

Purpose

Mammalian expression vector for miRFP670

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

miRFP670

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pKCyR5

Plasmid

#149463

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBLxR5

Plasmid

#167504

Purpose

Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLPC FLAG hRap1 delta MC

Plasmid

#13254

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta MC (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Mutation

contains hRap1 aa2-131 + 265-399 and the point mu…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pFLtR5

Plasmid

#167505

Purpose

Broad-Host range expression vector, RSF1010 origin, TetR repressor, PLtetO-1 promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TetR-RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLtetO-1

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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PL-452 C-Flag-4C

Plasmid

#19180

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

FLAG tetracysteine tag

Use

Cre/Lox

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pFLlR5

Plasmid

#149481

Purpose

Broad-Host range expression vector, RSF1010 origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LacI-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLlacO-1

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFVR5

Plasmid

#149489

Purpose

Broad-Host range expression vector, RSF1010 origin, VanR(AM) repressor, PVanCC promoter, mRFP, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFD-FKBP12

Plasmid

#137108

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

FKBP12

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFCyR5

Plasmid

#149480

Purpose

Broad-Host range expression vector, RSF1010 origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFPcR5

Plasmid

#149484

Purpose

Broad-Host range expression vector, RSF1010 origin, PcaU(AM) repressor, P3B5 promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

PcaU(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

P3B5B

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2-HA-LRR-NELC492A-C (B3-B4)

Plasmid

#211862

Purpose

Mutant E3-DART ligase in domestication vector

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

HA

Mutation

C492A

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCB6-ZONAB-A

Plasmid

#136641

Purpose

Expression of ZONAB isoform A in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al EMBO J. 2000 May 2;19(9):2024-33. doi: 1

Insert

ZONAB isoform A (YBX3 Canine)

Expression

Mammalian

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDL

Plasmid

#208978

Purpose

Dual-luciferase plasmid to measure enhancer activity in plants

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2024 Mar 21:koae088. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBac{Mhc-453-GFP,attB,w+}

Plasmid

#182199

Purpose

EGFP driven by 453bp enhancer of Mhc gene

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-Map7emtb

Plasmid

#46078

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7emtb

Tags

GFP

Expression

Mammalian

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0245

Plasmid

#162284

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pFCiK5

Plasmid

#167514

Purpose

Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mKelly2, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinRAM-mKelly2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQDKN0729

Plasmid

#162316

Purpose

TEV protease plasmid for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

T7prom-RBS-NET-TEVprotease

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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p18S.1 G693A

Plasmid

#51728

Purpose

transcribes pactamycin resistant 18S

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

18S

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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