We narrowed to 8,583 results for: construct
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Plasmid#124546PurposeCloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertWT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro
ExpressionMammalianMutationWT, WTPromoterMinimal ADEAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-CYFIP2.GFP
Plasmid#109140PurposeGFP-tagged CYFIP2 expression constructDepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F2bbs kan GCANNNAGGk
Plasmid#18752DepositorInsert3 tandem zinc fingers
ExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…Available SinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOPS0384
Plasmid#133234PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSYN-VWF-039
Plasmid#120292PurposeVWF expression construct expressing D'D3 domains with C1099A/C1142A mutations in D3 domain as an Fc fusion proteinDepositorInsertVWF D1-D2-D'-D3-Fc
TagsFcExpressionMammalianMutationC1099A/C1142APromoterCMVAvailable SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPS0385
Plasmid#133235PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), gusA (pOGG083) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0386
Plasmid#133236PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), celB (pOGG050) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0383
Plasmid#133233PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0380
Plasmid#133230PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom0.6
Plasmid#87353PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 0.6 kbpDepositorInsertMCT2 (SLC16A7 Human)
UseLuciferaseExpressionBacterial and MammalianPromoter0.6 kbp proximal promoter of human MCT2 (nested d…Available SinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1.1
Plasmid#32467DepositorInsertG-GECO1.1
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…Available SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-KD-mAbl
Plasmid#69028PurposeRetorviral vector encoding Kinase Defective mouse c-AblDepositorInsertmouse c-Abl Type VI - kinase defective
UseRetroviralTagsFLAG tag inserted at Internal SalI site (2934 bp)ExpressionMammalianMutationThe KD (kinase defective) mutation is at Lysine 2…Available SinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
DNMT3b KO Hyg
Plasmid#16650DepositorInsertDNMT3b knock-out construct (DNMT3B Human)
ExpressionBacterialAvailable SinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj10a-IRES-EGFP
Plasmid#164947PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and EGFPDepositorInsertkcnj10a (kcnj10a Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPPromoteractinb (actb2) promoterAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgULK1-1
Plasmid#109004PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No4
Plasmid#194899PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-4 targeting the promoter of human MECP2DepositorInsertsgRNA targeting human MECP2 promoter No.4
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
ath5:H2B-RFP
Plasmid#105960Purposetransgenesis, appears rather late, likely due to long RFP maturation time but is brighter than most other Ath5 constructDepositorAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-tetR-T2A-tSapphire
Plasmid#176312Purposeconstruct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-SapphireDepositorInsertstetR
T-sapphire
UseLentiviralAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330_Tuba1b sgRNA / hSpCas9
Plasmid#172835PurposeMammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP1054
Plasmid#119241Purposeconstruction intermediateDepositorTypeEmpty backboneExpressionBacterialAvailable SinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only