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FusX DdCBE DddA6 1397N

Plasmid

#221934

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddA6 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

DddA: Q1310R, S1330I, T1380I

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX DdCBE DddA11 1397N

Plasmid

#221935

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddA11 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

DddA: S1330I, A1341V, N1342S, E1370K, T1380I

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE DddA6-11 1397C

Plasmid

#221938

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6/11 1397C

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

TALE: Q231R, W232G, S233A | DddA: T1413I

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMK-N. tabacum trnI/trnA

Plasmid

#123579

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

N. tabacum trnI/trnA integration site

Use

Chloroplast transformation vector

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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MitoCKAR

Plasmid

#14872

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Tags

CFP, TOM20(1-33), and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pRec

Plasmid

#104572

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR, lacI

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-mRuby2

Plasmid

#89686

Purpose

pCAG promoter driving mRuby2

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGWT35S-RBCS1a(1–79)-TagRFP

Plasmid

#182586

Purpose

Transient expression of RBCS1a(1–79)-TagRFP in plant cell (Plastid/Chloroplast)

Depositor

Yutaka Kodama

Article

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

RBCS1a(1–79)

Tags

TagRFP

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pNuc-rxRFP1.1

Plasmid

#68131

Purpose

redox-sensitive red fluorescent protein rxRFP1.1, nuclear localization

Depositor

Huiwang Ai

Article

Fan et al Anal Bioanal Chem. 2016 Jan 12.

Insert

rxRFP1.1 and nuclear localization sequence

Tags

His Tag and nuclear localization sequence

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pDSG321

Plasmid

#115598

Purpose

surface-displayed Nb3, used in composability experiments; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDest490-OPN-c

Plasmid

#17592

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-c (SPP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 1–93, 175–942

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pBP_sgRNA

Plasmid

#190128

Purpose

Level 0 MoClo plasmid for Golden Gate cloning of sgRNAs

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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BALF5-WT

Plasmid

#192454

Purpose

Expresses wild-type BALF5 (codon-optimized)

Depositor

Bill Sugden

Article

Rosemarie et al mBio. 2023 Feb 28;14(1):e0268622. doi: 1

Insert

BALF5 (WT) (BALF5 Epstein-Barr Virus)

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMM107

Plasmid

#127227

Purpose

35S::IPT::pinII terminator

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

IPT

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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GolgiCKAR

Plasmid

#14871

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Tags

CFP, YFP, and eNOS(1-33)

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pDSG319

Plasmid

#115596

Purpose

surface-displayed Ag3, used in composability experiments; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnq3

Plasmid

#159911

Purpose

Mutagenesis of Kcnq3

Depositor

Larry Zweifel

Insert

Kcnq3 (Kcnq3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pWS3806

Plasmid

#185747

Purpose

Sub-array spacer 2/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 2/4

Use

CRISPR

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b_pScaC-SNAP_6xHis

Plasmid

#228835

Purpose

E. coli expression of the SNAP-tagged ScaC passenger domain

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

SNAPtag-TEV-6xHis

Expression

Bacterial

Mutation

aa 33-223

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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LSSmKate2-N1

Plasmid

#54796

Purpose

Localization: N1 Cloning Vector, Excitation: 460, Emission: 605

Depositor

Michael Davidson, Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Type

Empty backbone

Tags

LSSmKate2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits