We narrowed to 11,390 results for: mpl
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Plasmid#222825PurposeBacteria expression of MBP-ATG13 (450-517) and ULK1 MIT domain fusion complex -6HisDepositorAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only
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MBP-ATG13 (363-517)-ULK1 (836-1050)-His
Plasmid#222824PurposeBacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain fusion complex -6HisDepositorAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-GST-ATG13 (363-517)-ULK1 (836-1050)
Plasmid#222822PurposeBacteria expression of His-GST -ATG13 C-terminal and ULK1 MIT domain fusion complexDepositorAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Hygro-P2A-HA-FKBP-WAPL
Plasmid#217666PurposeRepair template to endogenously tag WAPL with Hygro-P2A-HA-FKBPDepositorInsertWapI (WAPL Human)
TagsdTag (FKBP36V protein)ExpressionBacterial and MammalianPromoterLacAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_DelNTerm_160-371AA
Plasmid#221487PurposeNKX2-1 N-terminal truncation mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationDeletion of 1-159AA N-terminal domainPromoterEF1aAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
SUMO CRYGS 16-177 Q16C
Plasmid#217673PurposeN-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligationDepositorInsertCRYGS (CRYGS Human)
Tags6XHis and SUMOExpressionBacterialMutationGlutamine 16 changed to Cysteine, and amino acids…PromoterT7Available SinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP2-Flag
Plasmid#210344PurposeExpresses SARS-CoV-2 NSP2 in mammalian cellsDepositorInsertSARS-CoV-2 NSP2 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
TagsFlagExpressionMammalianMutationMany synonymous changes due to codon optimizationAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-Prf1-mock-ires-mCherry
Plasmid#209074PurposeTargeting vector for the mouse Prf1 locus to correct prf1 geneDepositorInsertPrf1-mock-ires-mCherry HDR template (parts of Prf1(S399Stop) with mutated PAM) (Prf1 Mouse)
UseCRISPRAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-EZH1-Y727F
Plasmid#203582PurposeExpresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.DepositorInsertEZH1 (EZH1 Human)
UseLentiviralTagsV5-taggedExpressionMammalianMutationchanged Tyrosine 727 to Phenylalanine for partial…PromoterCMVAvailable SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-EZH1-dPSET
Plasmid#203586PurposeExpresses V5-tagged mutant version of EZH1 with deletion of aa 744-747 and partially resistant to JQ-EZ-05 in mammalian cells.DepositorInsertEZH1 (EZH1 Human)
UseLentiviralTagsV5-taggedExpressionMammalianMutationdeletion of aa 744-747 and partial resistance to …PromoterCMVAvailable SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(MRPS26(15))-PGKpuro2ABFP-W
Plasmid#200499PurposeLentiviral vector expressing gRNA targeting human MRPS26DepositorInsertMRPS26(15) (MRPS26 Human)
UseLentiviralAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(MRPS26(12))-PGKpuro2ABFP-W
Plasmid#200498PurposeLentiviral vector expressing gRNA targeting human MRPS26DepositorInsertMRPS26(12) (MRPS26 Human)
UseLentiviralAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ARID1B(23))-PGKpuro2ABFP-W
Plasmid#200470PurposeLentiviral vector expressing gRNA targeting human ARID1BDepositorInsertARID1B(23) (ARID1B Human)
UseLentiviralAvailable SinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ARID1B(21))-PGKpuro2ABFP-W
Plasmid#200469PurposeLentiviral vector expressing gRNA targeting human ARID1BDepositorInsertARID1B(21) (ARID1B Human)
UseLentiviralAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-T7-hTP53-P0~670-FLuc
Plasmid#208640Purposefor in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.DepositorInsertTP53, UTR and partial CDS (TP53 Human)
UseLuciferase; In vitor transcription templateTagsFirefly Luciferase (F-Luc) and T7 promoterMutationUTR and partial CDS in-frame with F-LucPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-FR-2Strep
Plasmid#210316PurposeExpresses SARS-CoV-2 NSP4 in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
Tags2StrepExpressionMammalianMutationMany synonymous changes due to codon optimizationAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH1-CMV-FIP200-L324D/R543D/R548D-mCherry-Hygro
Plasmid#210856PurposeLentiviral expression vector encoding FIP200 mutant-mCherryDepositorAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mScarlet-H3C2
Plasmid#207781PurposeDonor template for mScarlet insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780DepositorInsertH3C2 Homology Arms flanking a mScarlet Tag (H3C2 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
rUBR5_2383-2442_pGEX6P1
Plasmid#209791PurposeBacterial expression of UBR5 Mlle domainDepositorInsertMlle domain of UBR5 (Ubr5 Rat)
TagsGSTExpressionBacterialMutationaa 2383-2442 onlyPromotertacAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5A→M_IDR + 5M→A_CR
Plasmid#194268PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with TDP-43 _CTD_5A→M_IDR + 5M→A_CRDepositorInsertTDP-43 (TARDBP Synthetic, Human)
Tags6XHistagExpressionBacterialMutationA297M, A367M, A381M, A388M, A391M M322A, M323A, M…PromoterT7Available SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ATG13 (363-517)-ULK1 (836-1050)-TSF
Plasmid#203549PurposeMammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complexDepositorAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TSF-ATG13 (450-517)-ULK1 (836-1050)
Plasmid#203548PurposeMammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complexDepositorAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)
Plasmid#203547PurposeMammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complexDepositorAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_EF1α-mCherry
Plasmid#197429PurposeHomology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5DepositorInsertEF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsGCN4 leucine zipper, LOV2, Zdk1, and mCherryAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_EF1α-EGFP
Plasmid#197430PurposeHomology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5DepositorInsertEF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsEGFP, GCN4 leucine zipper, LOV2, and Zdk1Available SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-6xHis-TEV-ORF9c
Plasmid#201023PurposeBacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertopen reading frame 9c
Tags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-FAS-COMP5AP-AviTag-9xHis
Plasmid#157106PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-GST-GGSG-DmBam_13-36_AG
Plasmid#148827PurposeInsect Expression of DmBam_13-36DepositorInsertDmBam_13-36 (bam Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-MBP-HsNot9-V181E-shRNA2res_AG
Plasmid#148829PurposeMammalian Expression of HsNot9-V181E-shRNA2resDepositorInsertHsNot9-V181E-shRNA2res (CNOT9 Human)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1467-1717_AE
Plasmid#148598PurposeInsect Expression of DmNot1_1467-1717DepositorInsertDmNot1_1467-1717 (Not1 Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only