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pGEX SH2-B-SH2 RA

Plasmid

#46480

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2-B RK (SH2B1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 515-639; R555A mutation in t…

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Sck-SH2

Plasmid

#46478

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Sck/ShcB (Shc2 Mouse)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 464-573

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Socs7-SH2

Plasmid

#46509

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Socs7 (SOCS4 Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 280-386

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Nsp3-SH2

Plasmid

#46461

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nsp3 (SH2D3C Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 212-330

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SH2D5-SH2

Plasmid

#46483

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2D5 (Sh2d5 Mouse)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 290-399

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pLJD vector

Plasmid

#34595

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
HA-hAtg13

Plasmid

#31967

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

Atg13 (ATG13 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
rEc.13

Bacterial Strain

#69494

Purpose

Recoded strain from MG1655 with prfA

Depositor

Michael Jewett

Article

Hong et al ACS Synth Biol. 2014 Jun 20;3(6):398-409

Bacterial Resistance

Ampicillin

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-EML4-ALK variant 3a I1171N/D1203N

Plasmid

#183833

Purpose

Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cells

Depositor

Aaron Hata

Article

Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4

Inserts

ALK Receptor Tyrosine Kinase (ALK Human)
EMAP Like 4 (EML4 Human)

Use

Lentiviral

Tags

The fusion protein of EML4 exon 1-6a and ALK exon…

Expression

Mammalian

Mutation

I1171N/D1203N

Promoter

CMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1-sgAAVS1

Plasmid

#83906

Purpose

stable knockout

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

adeno-associated virus integration site 1 (AAVS1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSpp1-is3

Plasmid

#58247

Purpose

Expression vector producing isoform 3 derived mouse osteopontin (EGFP tagged)

Depositor

Georgios Stathopoulos

Article

Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

Insert

Spp1 isoform 3 (Spp1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-SomaGCaMP7

Plasmid

#182942

Purpose

Cre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-GCaMP7

Use

AAV

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBS10-ribE-dddAI-FLAG

Plasmid

#186562

Purpose

Regulated expression in Firmicutes of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Tags

FLAG

Expression

Bacterial

Promoter

pSpac(hy)

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPSV39-dddAI-FLAG

Plasmid

#186558

Purpose

Broad host range regulated expression of the DddA with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Tags

FLAG

Expression

Bacterial

Promoter

lacUV5

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_LacZ_sgRNA

Plasmid

#155108

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LacZ

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

LacZ_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJC-TALE-hairy

Plasmid

#46145

Depositor

David Stern

Article

Crocker et al Nat Methods. 2013 Aug;10(8):762-7. doi:

Type

Empty backbone

Tags

Hairy Repression Domain

Expression

Insect

Promoter

hsp70

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23106-BCD2-E0040m-B0015)

Plasmid

#197672

Purpose

Expresses GFP under the control of J23106 and BCD2

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23100-B0032m-E0040m-B0015)

Plasmid

#197657

Purpose

Expresses GFP under the control of J23100 and B0032m

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23100-B0033m-E0040m-B0015)

Plasmid

#197658

Purpose

Expresses GFP under the control of J23100 and B0033m

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23103-B0032m-E0040m-B0015)

Plasmid

#197663

Purpose

Expresses GFP under the control of J23103 and B0032m

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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