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SMX DEST vector

Plasmid

#62399

Purpose

Empty Backbone

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-ITGB3BP

Plasmid

#31211

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

ITGB3BP (ITGB3BP Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago Chi-a

Plasmid

#14075

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-a

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-a

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago Chi-b

Plasmid

#14085

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-b

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-b

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago Chi-c

Plasmid

#14086

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-c

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-c

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pEF5/FRT-DEST EVC2-TEV-YFP-Flag

Plasmid

#41004

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Tags

TEV-YFP-Flag

Expression

Mammalian

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

Plasmid

#113694

Purpose

U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) PHLDB1

Plasmid

#107503

Purpose

Expresses PHLDB1, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

PHLDB1 (PHLDB1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Nucleotide changes 1236 T>C, 1365 C>T, 1371…

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) eGFP

Plasmid

#107505

Purpose

Expresses eGFP, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

Plasmid

#69219

Purpose

Expresses wild type SpCas9 fused to Zif268 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

Zif268 (Egr1 Mouse)

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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hu-TLR4 299 SNP flag pLenti 4/to/v5 pDEST

Plasmid

#42650

Purpose

destination vector for lentivirus-mediated expression of human TLR4 299 SNP in mammalian cells

Depositor

Scott Friedman

Article

Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

Insert

TLR4 299 SNP (TLR4 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D299G

Promoter

CMV/TO

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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FUW-TetON-GFP

Plasmid

#115495

Purpose

Lentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)

Depositor

Catherine Verfaillie

Article

Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

Insert

eGFP

Use

Lentiviral

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH1_p.R132H

Plasmid

#81686

Purpose

Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway Cloning

Mutation

R132H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RELB_WT

Plasmid

#82232

Purpose

Gateway Donor vector containing RELB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RELB (RELB Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HIF1A_WT

Plasmid

#82129

Purpose

Gateway Donor vector containing HIF1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HIF1A (HIF1A Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-human-UBDUB-gRNA-puromycin-IRES-CFP

Pooled Library

#171531

Purpose

Knockout library targeting ubiquitination and de-ubiquitination complex genes.

Depositor

Chunliang Li

Article

Wright et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Expression

Mammalian

Species

Homo sapiens

Use

Lentiviral

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only
Human Brunello kinome pooled library, guides 5-8 in lentiGuide-Puro backbone

Pooled Library

#75313

Purpose

Human sgRNA library in backbone lentiGuide-Puro targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223_MAX_WT

Plasmid

#82888

Purpose

Gateway Donor vector containing MAX, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAX (MAX Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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mouse Oct4-GFP GOF18 transgenic reporter

Plasmid

#60527

Purpose

BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

GFPpA

Expression

Mammalian

Promoter

Oct4

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-YAP1-shRNA1

Plasmid

#176846

Purpose

lentiviral vector for doxycycline-inducible human YAP1 knockdown

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

YAP1-Sh1 (YAP1 Human)

Use

Lentiviral

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-mTurquoise2

Plasmid

#215539

Purpose

Expression vector with the mTurquoise2 fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

mTurquoise2

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPB.DEST-Illusia

Plasmid

#215444

Purpose

Piggybac expression vector with Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia

Use

Transposon-based stable expression

Expression

Mammalian

Promoter

CAG

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDest17_FL Gcn4

Plasmid

#231855

Purpose

Bacterial expression of N-terminally 6His tagged FL Gcn4

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-pDEST_BirA_Flag

Plasmid

#227960

Purpose

BioID experiment

Depositor

Alessandro Ori

Article

Ghoochani et al Cell. 2026 Feb 5;189(3):765-782.e31. doi

Insert

BirA

Expression

Mammalian

Mutation

R118G

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-NUDT5

Plasmid

#219955

Purpose

For protein purification from Ecoli

Depositor

Karla Feijs

Article

Weixler et al Commun Biol. 2025 Mar 18;8(1):453. doi:

Insert

NUDT5 (NUDT5 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST40-mPTER

Plasmid

#224864

Purpose

Mammalian expression mouse PTER protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

mouse PTER (Pter Mouse)

Tags

C-term v5 and His tag

Expression

Mammalian

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC Destination

Plasmid

#207675

Purpose

Trio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).

Depositor

Ramon Gonzalez

Article

Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

CymRC, PhlF, VanCC

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST40-CES2A-ΔC

Plasmid

#200554

Purpose

Mammalian expression of secreted mouse CES2A

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)

Tags

6xHis, Flag, and V5

Expression

Mammalian

Promoter

CMV promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:miRFP670-cetn4

Plasmid

#192357

Purpose

zfCETN4 tagged with miRFP670, expressed under the UAS promoter

Depositor

Darren Gilmour

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

CETN4 (cetn4 Zebrafish)

Tags

miRFP670

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_BH3-4E

Plasmid

#187144

Purpose

mammalian cell expression of FLAG-HUWE1 V1976E/V1780E/L1983E/M1987E

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

V1976E/V1780E/L1983E/M1987E

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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