We narrowed to 18,167 results for: STO;

1. pETM41-EcLonA

   Plasmid

   #38330

   Depositor

   Florian Freimoser

   Article

   Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

   Insert

   Lon protease fragment (lon Escherichia coli)
   

   Tags

   His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   Polyphosphate binding fragment of Lon protease. …

   Promoter

   T7

   Available Since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP

   Plasmid

   #114729

   Purpose

   3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc gene

   Depositor

   Martine Roussel

   Article

   Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

   Insert

   sgRNA M5, sgRNA M7, sgRNA M9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. KTK_251

   Plasmid

   #180546

   Purpose

   Entry vector containing mScarlet

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   mScarlet
   

   Expression

   Bacterial

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCellFree_G03 STAT3

   Plasmid

   #67120

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pmcsg7-mCRT(WT)

   Plasmid

   #83505

   Purpose

   Bacterial expression of Calreticulin

   Depositor

   Malini Raghavan

   Article

   Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

   Insert

   CALR (Calr Mouse)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pET-28a-His6-SUMO-VAMP2

   Plasmid

   #135551

   Purpose

   Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP2
   

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCellFree_G03 ISG15

   Plasmid

   #67054

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   ISG15 (ISG15 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA-NES-ZapCY2

   Plasmid

   #59016

   Purpose

   Cytosolic zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

   Insert

   NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
   

   Tags

   Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pET28a Gx-NL3

   Plasmid

   #169108

   Purpose

   Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - NanoLuc - NanoLuc - NanoLuc
   

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. EYFP-Clathrin-19

    Plasmid

    #56584

    Purpose

    Localization: Clathrin Vesicles, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Clathrin (CLTB Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pCellFree_G03 GATA1

    Plasmid

    #67068

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GATA1 (GATA1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCfB3020(gRNA X-2)

    Plasmid

    #73282

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCellFree_G03 TNF

    Plasmid

    #67062

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TNF (TNF Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pCR-Blunt II-TOPO-PTPRD

    Plasmid

    #29705

    Depositor

    Todd Waldman

    Article

    Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

    Insert

    Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
    

    Use

    Cloning vector

    Mutation

    none

    Available Since

    May 26, 2011

    Availability

    Academic Institutions and Nonprofits only

15. FLAG HDAC5 S259A

    Plasmid

    #32214

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser259Ala

    Promoter

    CMV

    Available Since

    Nov. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pmirGLO-Smad4-1719

    Plasmid

    #49383

    Purpose

    Insert is a fragment of the Smad4 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.

    Depositor

    Martine Roussel

    Article

    Murphy et al Cancer Res. 2013 Oct 21.

    Insert

    Smad family member 4 (SMAD4 Mouse, Human)
    

    Use

    Luciferase

    Promoter

    Human phosphoglycerate kinase (PGK) promoter

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB3042(gRNA X-4)

    Plasmid

    #73284

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. mVenus-CENPB-N-22

    Plasmid

    #56325

    Purpose

    Localization: Nucleus/Centromeres, Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    CENPB (CENPB Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    aa 1-169 of CENPB (DNA Binding Domain)

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCDNA3_PKA-CαN (K29,30A)-mVenus

    Plasmid

    #168485

    Purpose

    Mammalian expression of the K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.

    Depositor

    Haining Zhong

    Article

    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

    Insert

    the K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
    

    Expression

    Mammalian

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. STAGR_SAMScaffold_hH1

    Plasmid

    #102847

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert SAM_hH1
    

    Use

    Pcr template for stagr inserts

    Promoter

    U6

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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